Structure of PDB 8j83 Chain B

Receptor sequence

>8j83B (length=557) Species: 272630 (Methylorubrum extorquens AM1)

SFAHPGRGRNVARAVPKGRQVDPHAKVEIEELLGTRPRQRDLLIEHLHLI
QDTYGQISADHLAALADEMSLAFAEVFETATFYAHFDVVKEGEADIPRLT
IRVCDSITCAMFGADELLETLQRELASDAVRVVRAPCVGLCDHAPAVEVG
HNFLHRADLASVRAAVEAEDTHAHIPTYVDYDAYRAGGGYATLERLRSGE
LPVDDVLKVLDDGGLRGLGGAGFPTGRKWRSVRGEPGPRLMAVNGDEGEP
GTFKDQLYLNTDPHRFLEGMLIGAHVVEAADVYIYLRDEYPISREILARE
IAKLPEGTRIHLRRGAGAYICGEESSLIESLEGKRGLPRHKPPFPFQVGL
FNRPTLINNIETLFWVRDLIERGAEWWKSHGRNGRVGLRSYSVSGRVKEP
GVKLAPAGLTIQELIDEYCGGISDGHSFAAYLPGGASGGILPASMNDIPL
DFGTLEKYGCFIGSAAVVILSDQDDVRGAALNLMKFFEDESCGQCTPCRS
GTQKARMLMENGVWDTDLLGELAQCMRDASICGLGQAASNPVSTVIKYFP
DLFPEPR

3D structure

• PDB: 8j83 Structure and function relationship of formate dehydrogenases: an overview of recent progress.
• Chain: B
• Resolution: 2.4 Å

Enzymatic activity

• Enzyme Commision number: 1.2.1.2: Transferred entry: 1.17.1.9.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FMN	B	G226 G228 K237 N253 E256 Y329 G332 E333 N368 N369	G217 G219 K228 N244 E247 Y319 G322 E323 N358 N359
BS02	SF4	B	S501 C502 G503 Q504 C505 C508 I541 C542 L544 G545	S491 C492 G493 Q494 C495 C498 I531 C532 L534 G535
BS03	FES	B	C113 S115 T117 C118 C146 V147 L149 C150	C104 S106 T108 C109 C137 V138 L140 C141

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0008137 NADH dehydrogenase (ubiquinone) activity
• GO:0010181 FMN binding
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0051537 2 iron, 2 sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:1902600 proton transmembrane transport

External links

• PDB: RCSB:8j83, PDBe:8j83, PDBj:8j83
• PDBsum: 8j83
• PubMed: 37668215
• UniProt: C5ATT6