Structure of PDB 8j1j Chain B

Receptor sequence

>8j1jB (length=387) Species: 1765684 (Sulfoacidibacillus thermotolerans) [Search protein sequence]

MIKVYRYEIVKPLDLDWKEFGTILRQLQQETRFALNKATQLAWEWMGYSS
DYKDNHGEILGYTNVHGYAYHTIKTKAYRLNSGNLSQTIKRATDRFKAYQ
KEILRGDMSIPSYKRDIPLDLIKENISVNRMNHGDYIASLSLLSNPAKQE
MNVKRKISVIIIVRGAGKTIMDRILSGEYQVHASQIIHDDRKNKWYLNIS
YDFEPQTRVLDLNKIMGIDLGVAVAAYMAFQHTPARYKLEGGEIENFRRQ
VESRRISMLKPIEQLRDKIANFRDTTNHRYSRYIVDMAIKMGCGTIQMED
LDIGSRFLQNWTYYDLQQKIIYKAEEAGIKVIKIDPQYTSQRCSECGNID
GQAIFKCRACGYEANADYNAARNIAIPNIDKIIAESI

3D structure

PDB

8j1j Minimal and most efficient genome editing Cas enzyme

Chain

Resolution

2.91 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	dna	B	V4 R6 P240 R242	V4 R6 P234 R236
BS02	rna	B	K3 N70 H72 G73 Y76 S92 K96 K103 K107 K129 R197 P240 G248 E249 N252 F253 R255 Q256 S259 R260 K293 N296	K3 N64 H66 G67 Y70 S86 K90 K97 K101 K123 R191 P234 G242 E243 N246 F247 R249 Q250 S253 R254 K268 N271
BS03	ZN	B	C372 C375 C391 C394	C343 C346 C357 C360

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003723	RNA binding
GO:0004519	endonuclease activity
GO:0046872	metal ion binding

View graph for
Molecular Function

External links

PDB	RCSB:8j1j, PDBe:8j1j, PDBj:8j1j
PDBsum	8j1j
PubMed
UniProt	A0A2U3D0N8\|CS12F_SULT2 CRISPR-associated endodeoxyribonuclease Cas12f1 (Gene Name=cas12f1)

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