Structure of PDB 8j12 Chain B

Receptor sequence
>8j12B (length=385) Species: 1765684 (Sulfoacidibacillus thermotolerans) [Search protein sequence]
MIKVYRYEIVKPLDLDWKEFGTILRQLQQETRFALNKATQLAWEWMGFSS
DYKDNLGYTNVHGYAYHTIKTKAYRLNSGNLSQTIKRATDRFKAYQKEIL
RGDMSIPSYKRDIPLDLIKENISVNRMNHGDYIASLSLLSNPAKQEMNVK
RKISVIIIVRGAGKTIMDRILSGEYQVSASQIIHDDRKNKWYLNISYDFE
PQTRVLDLNKIMGIDLGVAVAVYMAFQHTPARYKLEGGEIENFRRQVESR
RISMLRQKPIEQLRDKIANFRDTTNHRYSRYIVDMAIKEGCGTIQMEDLD
IGSRFLQNWTYYDLQQKIIYKAEEAGIKVIKIDPQYTSQRCSECGNIDGQ
AIFKCRACGYEANADYNAARNIAIPNIDKIIAESI
3D structure
PDB8j12 Minimal and most efficient genome editing Cas enzyme
ChainB
Resolution3.08 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.-.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B R6 P240 R242 R6 P230 R232
BS02 rna B K3 H72 G73 Y76 S92 K96 I176 R197 Y207 F209 P240 E249 N252 F253 Q256 S259 R260 K293 N296 F297 K3 H62 G63 Y66 S82 K86 I166 R187 Y197 F199 P230 E239 N242 F243 Q246 S249 R250 K266 N269 F270
BS03 ZN B C372 C375 C391 C394 C341 C344 C355 C358
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0046872 metal ion binding

View graph for
Molecular Function
External links
PDB RCSB:8j12, PDBe:8j12, PDBj:8j12
PDBsum8j12
PubMed
UniProtA0A2U3D0N8|CS12F_SULT2 CRISPR-associated endodeoxyribonuclease Cas12f1 (Gene Name=cas12f1)

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