Structure of PDB 8j0e Chain B

Receptor sequence
>8j0eB (length=269) Species: 3702 (Arabidopsis thaliana) [Search protein sequence]
DRSRAFAKDVKRIVVKVGTAVVTGKGGRLALGRLGAICEQLAELNSDGFE
VILVSSGAVGLGRQRLRYRQLVNSSFADLQKPQMELDGKACAGVGQSSLM
AYYETMFDQLDVTVAQMLVTDSSFRDKDFRKQLSETVKAMLRMRVIPVFN
ENDAISTRFWDNDSLAALLSLELKADLLILLSDVEGLYTGPPSDSTSKLI
HTFIKEKHQDEITFGEKRGGMTAKVKAAVNAAYGGVPVIITSGYAAENIS
KVLRGLRVGTLFHQDAHLW
3D structure
PDB8j0e GK monomer complexes with catalytic intermediate
ChainB
Resolution3.4 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 1.2.1.41: glutamate-5-semialdehyde dehydrogenase.
2.7.2.11: glutamate 5-kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 peptide B V76 S78 F80 V72 S74 F76
BS02 peptide B Y72 L75 V76 F80 Q84 Y68 L71 V72 F76 Q80
BS03 TKC B K20 G22 T23 A24 S60 G61 V63 D157 N176 V198 P206 K231 R236 G237 G238 M239 K16 G18 T19 A20 S56 G57 V59 D153 N162 V184 P192 K217 R218 G219 G220 M221
BS04 MG B D177 G237 D163 G219
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0004349 glutamate 5-kinase activity
GO:0004350 glutamate-5-semialdehyde dehydrogenase activity
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016491 oxidoreductase activity
GO:0016620 oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
Biological Process
GO:0006561 proline biosynthetic process
GO:0009084 glutamine family amino acid biosynthetic process
GO:0009555 pollen development
GO:0009793 embryo development ending in seed dormancy
GO:0016310 phosphorylation
GO:0055129 L-proline biosynthetic process
Cellular Component
GO:0005737 cytoplasm
GO:0009506 plasmodesma
GO:0009507 chloroplast

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8j0e, PDBe:8j0e, PDBj:8j0e
PDBsum8j0e
PubMed
UniProtP54888|P5CS2_ARATH Delta-1-pyrroline-5-carboxylate synthase B (Gene Name=P5CSB)

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