Structure of PDB 8ip1 Chain B

Receptor sequence

>8ip1B (length=503) Species: 562 (Escherichia coli)

TQRFDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKS
LWHNVENRQLDAQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYN
DAGVDTKQLDLGFAGFRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLS
ARGLAIDTYTDSKEEFPDFTAFWFDTVKPGATTFTVYALLDSASITGAYK
FTIHCEKSQVIMDVENHLYARKDIKQLGIAPMTSMFSCGTNERRMCDTIH
PQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNPKGFGLLQLDRD
FSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLNNIVCFWQ
PEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFSEGWAPGE
HYPEKWARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPID
GYRIQFDWYPTSDSTDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYV
DDR

3D structure

• PDB: 8ip1 Novel glycoside hydrolase family enzymes from Escherichia coli associating with osmo-regulated periplasmic glucan synthesis
• Chain: B
• Resolution: 2.06 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BGC	B	Y182 R197 E209 T278 N388 V391	Y137 R152 E164 T233 N343 V346
BS02	BGC	B	F112 L178 Y182 R184 S195 N388	F71 L133 Y137 R139 S150 N343
BS03	BGC	B	R184 M280 D300 R359 E385 T386 L387 N388	R139 M235 D255 R314 E340 T341 L342 N343
BS04	BGC	B	N82 F112	N41 F71
BS05	BGC	B	Q191 R359	Q146 R314
BS06	BGC	B	R162 D173 Y192	R117 D128 Y147
BS07	BGC	B	Y190 Q191 Y192	Y145 Q146 Y147
BS08	BGC	B	R171 R172	R126 R127

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0030246 carbohydrate binding

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0016051 carbohydrate biosynthetic process

Cellular Component

• GO:0042597 periplasmic space

External links

• PDB: RCSB:8ip1, PDBe:8ip1, PDBj:8ip1
• PDBsum: 8ip1
• PubMed: 37735577
• UniProt: P40120|OPGD_ECOLI Glucans biosynthesis protein D (Gene Name=mdoD)