Structure of PDB 8if8 Chain B

Receptor sequence

>8if8B (length=620) Species: 83332 (Mycobacterium tuberculosis H37Rv)

ARAREVLVALGQLAAAVVVAVGVAVVSLLAIARVEWPAFPSSNQLHALTT
VGQVGCLAGLVGIGWLWRHGRFRRLARLGGLVLVSAFTVVTLGMPLGATK
LYLFGISVDQQFRTEYLTRLTDTAALRDMTYIGLPPFYPPGWFWIGGRAA
ALTGTPAWEMFKPWAITSMAIAVAVALVLWWRMIRFEYALLVTVATAAVM
LAYSSPEPYAAMITVLLPPMLVLTWSGLGARDRQGWAAVVGAGVFLGFAA
TWYTLLVAYGAFTVVLMALLLAGSRLQSGIKAAVDPLCRLAVVGAIAAAI
GSTTWLPYLLRAARDPVSDTGSAQHYLPADGAALTFPMLQFSLLGAICLL
GTLWLVMRARSSAPAGALAIGVLAVYLWSLLSMLATLARTTLLSFRLQPT
LSVLLVAAGAFGFVEAVQALGKRGRGVIPMAAAIGLAGAIAFSQDIPDVL
RPDLTIAYTDTDGYGQRGDRRPPGSEKYYPAIDAAIRRVTGKRRDRTVVL
TADYSFLSYYPYWGFQGLTPHYANPLAQFDKRATQIDSWSGLSTADEFIA
ALDKLPWQPPTVFLMRHGAHNSYTLRLAQDVYPNQPNVRRYTVDLRTALF
ADPRFVVEDIGPFVLAIRKP

3D structure

• PDB: 8if8 Structure of the priming arabinosyltransferase AftA required for AG biosynthesis of Mycobacterium tuberculosis.
• Chain: B
• Resolution: 3.1 Å

Enzymatic activity

• Enzyme Commision number: 2.4.2.46: galactan 5-O-arabinofuranosyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	B	E134 Y523 L537	E115 Y504 L518

Gene Ontology

Molecular Function

• GO:0016757 glycosyltransferase activity
• GO:0035250 UDP-galactosyltransferase activity

Biological Process

• GO:0044038 cell wall macromolecule biosynthetic process
• GO:0045227 capsule polysaccharide biosynthetic process
• GO:0071555 cell wall organization
• GO:0071769 mycolate cell wall layer assembly

Cellular Component

• GO:0005886 plasma membrane
• GO:0009274 peptidoglycan-based cell wall
• GO:0016020 membrane

External links

• PDB: RCSB:8if8, PDBe:8if8, PDBj:8if8
• PDBsum: 8if8
• PubMed: 37252995
• UniProt: P9WN03|AFTA_MYCTU Galactan 5-O-arabinofuranosyltransferase (Gene Name=aftA)