Structure of PDB 8hp4 Chain B

Receptor sequence

>8hp4B (length=530) Species: 5482 (Candida tropicalis)

SEITLGRFFFERLHQLQVDTVFGLPGDFNLALLDKIYEVDGMRWAGNANE
LNAGYAADGYARVNPNGLAALVSTFGVGELSLTNAIAGSYSEHVGIINLV
GVPSLHHTLGNGDFTVFHRMFKNISQTSAFISDPNTAASEIDRCIRDAYV
YQRPVYIGLPSNLVDVKVPKSLLDKKIDLSLHPNEPESQAEVVETVEKFI
SEASNPVILVDACAIRHNCLKEVAELIAETQFPVFTTPMGKSSVDESNPR
FGGVYVGSLSSPDVKEAVESADLVLSVGAMLNVVEFHSDYTKIRQATFPG
VQMKEALQVLLKTVKKSVNPKYVPAPVPATPGNNDPVSQEYLWRKVSDWF
QEGDVIISETGTSAFGIVQSKFPKNAIGISQVLWGSIGYATGATCGAAMA
AQEIDPKKRVILFTGDGSLQLTVQEISTMCKWDCYNTYLYVLNNDGYTIE
RLIHGEKAQYNDIQPWNNLQLLPLFNAKKYETKRISTVGELNDLFTNKEF
AVPDRIRMVEIMLPVMDAPANLVAQAKQSA

3D structure

• PDB: 8hp4 Protein engineering of pyruvate decarboxylase to remove the rate-limiting bottleneck in the cascade pathway of tyrosol synthesis
• Chain: B
• Resolution: 2.52 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	TPP	B	P26 E51 V78	P25 E50 V77
BS02	TPP	B	T392 S416 I417 G445 G447 S448 N474 G476 Y477 T478 I479 E480	T362 S386 I387 G415 G417 S418 N444 G446 Y447 T448 I449 E450
BS03	MG	B	D446 N474 G476	D416 N444 G446

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0004737 pyruvate decarboxylase activity
• GO:0016831 carboxy-lyase activity
• GO:0030976 thiamine pyrophosphate binding
• GO:0046872 metal ion binding

Biological Process

• GO:0000949 aromatic amino acid family catabolic process to alcohol via Ehrlich pathway

Cellular Component

• GO:0005634 nucleus
• GO:0005829 cytosol

External links

• PDB: RCSB:8hp4, PDBe:8hp4, PDBj:8hp4
• PDBsum: 8hp4
• UniProt: C5MDS4