Structure of PDB 8hfd Chain B

Receptor sequence

>8hfdB (length=447) Species: 562 (Escherichia coli)

SFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVMDASGLVV
SPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVD
RASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVAG
IDNDFRDVNDWQFFKGAQKLGELGQPVLVHCENALICDELGEEAKREGRV
TAHDYVASRPVFTEVEAIRRVLYLAKVAGCRLHVCHVSSPEGVEEVTRAR
QEGQDVTCESCPHYFVLDTDQFEEIGTLAKCSPPIRDLENQKGMWEKLFN
GEIDCLVSDHSPCPPEMKAGNIMEAWGGIAGLQNCMDVMFDEAVQKRGMS
LPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQPNSSYVLTNDDLEY
RHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILKHQQ

3D structure

• PDB: 8hfd Crystal Structure of Allantoinase from Escherichia coli BL21: A Molecular Insight into a Role of the Active Site Loops in Catalysis.
• Chain: B
• Resolution: 2.07 Å

Enzymatic activity

• Enzyme Commision number: 3.5.2.5: allantoinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	B	H59 H61 K146 D315	H58 H60 K145 D309
BS02	ZN	B	K146 H186 H242	K145 H180 H236

Gene Ontology

Molecular Function

• GO:0004038 allantoinase activity
• GO:0008270 zinc ion binding
• GO:0016787 hydrolase activity
• GO:0016810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
• GO:0046872 metal ion binding
• GO:0050897 cobalt ion binding

Biological Process

• GO:0000256 allantoin catabolic process
• GO:0006144 purine nucleobase metabolic process
• GO:0006145 purine nucleobase catabolic process
• GO:0009442 allantoin assimilation pathway

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:8hfd, PDBe:8hfd, PDBj:8hfd
• PDBsum: 8hfd
• PubMed: 36677881
• UniProt: P77671|ALLB_ECOLI Allantoinase (Gene Name=allB)