Structure of PDB 8hbh Chain B

Receptor sequence
>8hbhB (length=577) Species: 9606 (Homo sapiens) [Search protein sequence]
MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYD
LVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQN
LDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGII
KTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKEESRISPYTFCKAFPF
HIIFDRDLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGIL
SHINTVFVLRSKEGLLDVISCLRLKGQMIYLPEADSILFLCSPSVMNLDD
LTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQLTLRA
LEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNA
FCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTV
SGLPEPCIHHARSICHLALDMMEIAGQVQVDGESVQITIGIHTGEVVTGV
IGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPQF
HLEHRGPVSMKGKKEPMQVWFLSRKNT
3D structure
PDB8hbh NO binds to the distal site of haem in the fully activated soluble guanylate cyclase.
ChainB
Resolution3.1 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 4.6.1.2: guanylate cyclase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 G2P B F424 E473 V547 S551 R552 K593 F392 E441 V515 S519 R520 K561
BS02 HEM B M1 Y2 F4 F74 H105 L108 S137 R139 L142 V146 I149 M1 Y2 F4 F74 H105 L108 S137 R139 L142 V146 I149
Gene Ontology
Molecular Function
GO:0004016 adenylate cyclase activity
GO:0004383 guanylate cyclase activity
GO:0005515 protein binding
GO:0005525 GTP binding
GO:0016829 lyase activity
GO:0016849 phosphorus-oxygen lyase activity
GO:0020037 heme binding
GO:0038023 signaling receptor activity
GO:0044877 protein-containing complex binding
GO:0046872 metal ion binding
GO:0047805 cytidylate cyclase activity
GO:0051879 Hsp90 protein binding
GO:0070026 nitric oxide binding
Biological Process
GO:0006182 cGMP biosynthetic process
GO:0007263 nitric oxide mediated signal transduction
GO:0008015 blood circulation
GO:0009190 cyclic nucleotide biosynthetic process
GO:0019934 cGMP-mediated signaling
GO:0035556 intracellular signal transduction
GO:0038060 nitric oxide-cGMP-mediated signaling
GO:0070482 response to oxygen levels
GO:0071732 cellular response to nitric oxide
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0008074 guanylate cyclase complex, soluble
GO:0032991 protein-containing complex
GO:0098831 presynaptic active zone cytoplasmic component
GO:0098978 glutamatergic synapse

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8hbh, PDBe:8hbh, PDBj:8hbh
PDBsum8hbh
PubMed36972843
UniProtQ02153|GCYB1_HUMAN Guanylate cyclase soluble subunit beta-1 (Gene Name=GUCY1B1)

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