Structure of PDB 8fht Chain B

Receptor sequence
>8fhtB (length=468) Species: 9606 (Homo sapiens) [Search protein sequence]
VNGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLS
ISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGF
AETVRDLLQEYGAPIVDPINDIRIIGVVSVTVLLAISLAGMEWESKAQVL
FFLVIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVPDWRGPDG
TFFGMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLA
ISATIGSCVVRDASGVLNDTVTPGWGACEGLACSYGWNFTECTQQHSCHY
GLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQL
YPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLC
SYALINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWA
ALIAIGVVLFLLLYVIYK
3D structure
PDB8fht Structure and thiazide inhibition mechanism of the human Na-Cl cotransporter.
ChainB
Resolution3.02 Å
3D
structure
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Enzymatic activity
Enzyme Commision number ?
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 NA B L148 W151 G152 A464 S467 S468 L10 W13 G14 A326 S329 S330
BS02 CL B G353 L355 Y540 G215 L217 Y402
Gene Ontology
Molecular Function
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008511 sodium:potassium:chloride symporter activity
GO:0015081 sodium ion transmembrane transporter activity
GO:0015293 symporter activity
GO:0015377 chloride:monoatomic cation symporter activity
GO:0015378 sodium:chloride symporter activity
GO:0022857 transmembrane transporter activity
GO:0046873 metal ion transmembrane transporter activity
Biological Process
GO:0002021 response to dietary excess
GO:0006811 monoatomic ion transport
GO:0006814 sodium ion transport
GO:0006884 cell volume homeostasis
GO:0007165 signal transduction
GO:0035725 sodium ion transmembrane transport
GO:0055064 chloride ion homeostasis
GO:0055075 potassium ion homeostasis
GO:0055078 sodium ion homeostasis
GO:0055085 transmembrane transport
GO:0070294 renal sodium ion absorption
GO:1902476 chloride transmembrane transport
GO:1904044 response to aldosterone
GO:1990573 potassium ion import across plasma membrane
Cellular Component
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0016324 apical plasma membrane
GO:0070062 extracellular exosome

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8fht, PDBe:8fht, PDBj:8fht
PDBsum8fht
PubMed36792826
UniProtP55017|S12A3_HUMAN Solute carrier family 12 member 3 (Gene Name=SLC12A3)

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