Structure of PDB 8fho Chain B

Receptor sequence

>8fhoB (length=847) Species: 7955 (Danio rerio)

KEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVT
VTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVG
VAMHTVGFAATVRDLLQEYGAPIVDPINDIRIIGVVSVTVLLAISLAGME
WESKAQVLFFLVIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLV
PDWRGPDGTFFGMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIF
WTTISYLAISATIGSCVVRDASGVLNDTVTPGWGACEGLACSYGWNFTEC
TQQHSCHYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVF
QCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAP
IISNFFLCSYALINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVI
MFLLTWWAALIAIGVVLFLLLYVIYSYNLALSYSVGLNEVEDHIKNYRPQ
CLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPELQLIAN
GHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVGFKK
NWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKEEQATTIFQS
EQGKKTIDIYWLFDDGGLTLLIPYLLGRKRRWSKCKIRVFVGGQINRMDQ
ERKAIISLLSKFRLGFHEVHILPDINQNPRAEHTKRFEDMIAPFRLNDGF
KDEATVNEMRRDCPWKISDEEITKNRVKSLRQVRLNEIVLDYSRDAALIV
ITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLTFYCQ

3D structure

• PDB: 8fho Structure and thiazide inhibition mechanism of the human Na-Cl cotransporter.
• Chain: B
• Resolution: 2.95 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	B	H762 W835 F837 D838 F886 I979 R981 K982 K984 R1009 N1011 Q1012 N1014 F1018	H618 W661 F663 D664 F712 I805 R807 K808 K810 R835 N837 Q838 N840 F844
BS02	ATP	B	L648 R655 V677 L717 G741 K743 K744 N781	L504 R511 V533 L573 G597 K599 K600 N637
BS03	XZF	B	N149 F223 N227 M233 P349 T352 L355 A356 C472 S533 F536	N19 F93 N97 M103 P219 T222 L225 A226 C342 S403 F406
BS04	NA	B	L148 W151 A464 S467 S468	L18 W21 A334 S337 S338

Gene Ontology

Molecular Function

• GO:0015377 chloride:monoatomic cation symporter activity
• GO:0022857 transmembrane transporter activity

Biological Process

• GO:0006811 monoatomic ion transport
• GO:0055085 transmembrane transport

Cellular Component

• GO:0016020 membrane

External links

• PDB: RCSB:8fho, PDBe:8fho, PDBj:8fho
• PDBsum: 8fho
• PubMed: 36792826
• UniProt: P55017|S12A3_HUMAN Solute carrier family 12 member 3 (Gene Name=SLC12A3)