Structure of PDB 8fgb Chain B

Receptor sequence

>8fgbB (length=416) Species: 10116 (Rattus norvegicus)

RFLKVKNWETDVVLTDTLHLKSTLETGCTEHICMGSIMLPSQHTRVRTKD
QLFPLAKEFLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELI
YGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNK
GNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVQFT
EICIQQGWKAPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFD
WFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNS
RYNILEEVAKKMDLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHH
SATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTP
SFEYQPDPWNTHVWKG

3D structure

• PDB: 8fgb Potent, Selective, and Membrane Permeable 2-Amino-4-Substituted Pyridine-Based Neuronal Nitric Oxide Synthase Inhibitors.
• Chain: B
• Resolution: 1.91 Å

Enzymatic activity

• Enzyme Commision number: 1.14.13.39: nitric-oxide synthase (NADPH).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	H4B	B	F691 H692 Q693	F389 H390 Q391
BS02	HEM	B	H341 W409 R414 C415 F584 W587 W678 F704 Y706	H43 W107 R112 C113 F282 W285 W376 F402 Y404
BS03	H4B	B	S334 M336 R596 V677 W678	S36 M38 R294 V375 W376
BS04	V80	B	M336 V567 M570 F584 S585 W587 E592 Y706	M38 V265 M268 F282 S283 W285 E290 Y404
BS05	ZN	B	C326 C331	C28 C33

Gene Ontology

Molecular Function

• GO:0004517 nitric-oxide synthase activity

Biological Process

• GO:0006809 nitric oxide biosynthetic process

External links

• PDB: RCSB:8fgb, PDBe:8fgb, PDBj:8fgb
• PDBsum: 8fgb
• PubMed: 37433128
• UniProt: P29476|NOS1_RAT Nitric oxide synthase 1 (Gene Name=Nos1)