Structure of PDB 8fbm Chain B

Receptor sequence

>8fbmB (length=426) Species: 353152 (Cryptosporidium parvum Iowa II)

PHKFWNTQPVVQNDDSSSEYSFGPIEIEPDSFRKEIYKLPDGFSWFDCNL
WDIESQDFEDTYQLLKDHYVEDDDSQFRFNYSKEFLRWALCVPGQKKNWL
VGVRVNETKKMVGFISAIPIKVRIHNCIMNTSVVNFLCVHKKLRSKRLAP
VLIKEITRRIRCEKIFQSIYTCGKNITKPFTIGTYWHRIINVKKLLEAGF
IGIPRNMTMSSLIKYHRIPADKRIEGFRPSVDSDAEQICKLFENYFMKYK
DVSNETMNNLINYDEINHSKELGKQAYMKLDKIEDLQDKITIHQCFNVED
VKHYFTNIDKVIVTYVRENKNKEITDLFSFFIIESTVINNERFPTINIAY
SYFNIANTCSLKELFNEMLITAKNNNCDAFNTLDLMQNLQVIQDSKFIIG
TGRLRYYVFNWKIPQISPSNVGIILF

3D structure

• PDB: 8fbm Crystal structure of Cryptosporidium parvum N-myristoyltransferase with bound myristoyl-CoA
• Chain: B
• Resolution: 1.9 Å

Enzymatic activity

• Enzyme Commision number: 2.3.1.97: glycylpeptide N-tetradecanoyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MYA	B	H42 K43 F44 W45 Y109 V110 V174 L177 V179 R184 S185 R187 A189 P190 T197 I200 F206 Q207 S208 Y210 C212 K214 I216 F449	H2 K3 F4 W5 Y69 V70 V134 L137 V139 R144 S145 R147 A149 P150 T157 I160 F166 Q167 S168 Y170 C172 K174 I176 F409
BS02	XOF	B	V110 D112 F117 F119 Y121 Y225 S375 Y390 N421 G442 R443 L444 F466	V70 D72 F77 F79 Y81 Y185 S335 Y350 N381 G402 R403 L404 F426

Gene Ontology

Molecular Function

• GO:0004379 glycylpeptide N-tetradecanoyltransferase activity
• GO:0016746 acyltransferase activity

Biological Process

• GO:0006499 N-terminal protein myristoylation
• GO:0018008 N-terminal peptidyl-glycine N-myristoylation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:8fbm, PDBe:8fbm, PDBj:8fbm
• PDBsum: 8fbm
• PubMed: 37722671
• UniProt: Q5CV46