Structure of PDB 8evu Chain B

Receptor sequence

>8evuB (length=412) Species: 345073 (Vibrio cholerae O395)

LKKFLEDIEHHFEPGGKHEKWFALYEAAATLFYTPGLVTKRSSHVRDSVD
LKRIMIMVWLAVFPAMFWGMYNAGGQAIAALNHLYSGDQLAAIVAGNWHY
WLTEMLGGTMSSDAGWGSKMLLGATYFLPIYATVFIVGGFWEVLFCMVRK
HEVNEGFFVTSILFALIVPPTLPLWQAALGITFGVVVAKEVFGGTGRNFL
NPALAGRAFLFFAYPAQISGDLVWTAADGYSGATALSQWAQGGAGALINN
ATGQTITWMDAFIGNIPGSIGEVSTLALMIGAAFIVYMGIASWRIIGGVM
IGMILLSTLFNVIGSDTNAMFNMPWHWHLVLGGFAFGMFFMATDPVSASF
TNSGKWAYGILIGVMCVLIRVVNPAYPEGMMLAILFANLFAPLFDHVVVE
RNIKRRLARYGK

3D structure

• PDB: 8evu Novel cofactor binding motifs, electron transfer and ion pumping mechanisms of the respiratory complex NQR
• Chain: B
• Resolution: 2.5804 Å

Enzymatic activity

• Enzyme Commision number: 7.2.1.1: NADH:ubiquinone reductase (Na(+)-transporting).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FMN	B	I169 R209 F213 W226 T236 L238 S271 E274 G334 G335 E380 G381 M383 L384	I167 R207 F211 W224 T234 L236 S269 E272 G332 G333 E378 G379 M381 L382
BS02	RBF	B	M57 V60 V161 T162 L165 T197 G198 N200 N203 P204 A205 F342 M343 D346 P347 V348	M55 V58 V159 T160 L163 T195 G196 N198 N201 P202 A203 F340 M341 D344 P345 V346
BS03	UQ1	B	L33 K54 E157 G158 F159 F160	L31 K52 E155 G156 F157 F158
BS04	FMN	B	F213 P217 D223 A377	F211 P215 D221 A375

Gene Ontology

Molecular Function

• GO:0010181 FMN binding
• GO:0016655 oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
• GO:1902444 riboflavin binding

Biological Process

• GO:0006814 sodium ion transport
• GO:0022904 respiratory electron transport chain
• GO:0055085 transmembrane transport

Cellular Component

• GO:0005886 plasma membrane
• GO:0016020 membrane

External links

• PDB: RCSB:8evu, PDBe:8evu, PDBj:8evu
• PDBsum: 8evu
• UniProt: A5F5X0|NQRB_VIBC3 Na(+)-translocating NADH-quinone reductase subunit B (Gene Name=nqrB)