Structure of PDB 8eiy Chain B

Receptor sequence

>8eiyB (length=374) Species: 9796 (Equus caballus)

STAGKVIKCKAAVLWEEKKPFSIEEVEVAPPKAHEVRIKMVATGICRTDD
HVVSGTLVTPLPVIAGHEAAGIVESIGEGVTTVRPGDKVIPLFTPQCGKC
RVCKHPEGNFCLKNDLSMPRGTMQDGTSRFTCRGKPIHHFLGTSTFSQYT
VVDEISVAKIDAASPLEKVCLIGCGFSTGYGSAVKVAKVTQGSTCAVFGL
GGVGLSVIMGCKAAGAARIIGVDINKDKFAKAKEVGATECVNPQDYKKPI
QEVLTEMSNGGVDFSFEVIGRLDTMVTALSCCQEAYGVSVIVGVPPDSQN
LSMNPMLLLSGRTWKGAIFGGFKSKDSVPKLVADFMAKKFALDPLITHVL
PFEKINEGFDLLRSGESIRTILTF

3D structure

• PDB: 8eiy Structure of cobalt(II)-substituted S48T horse liver alcohol dehydrogenase at 2.55 Angstroms resolution
• Chain: B
• Resolution: 2.55 Å

Enzymatic activity

• Enzyme Commision number: 1.1.1.1: alcohol dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CO	B	C46 T48 H67 C174	C46 T48 H67 C174
BS02	ZN	B	C97 C100 C103 C111	C97 C100 C103 C111
BS03	NAI	B	R47 T48 H51 C174 T178 G199 G201 V203 D223 I224 I269 V292 G293 V294 I318 F319 R369	R47 T48 H51 C174 T178 G199 G201 V203 D223 I224 I269 V292 G293 V294 I318 F319 R369
BS04	CXF	B	T48 H67 F93 C174 V294	T48 H67 F93 C174 V294

Gene Ontology

Molecular Function

• GO:0004022 alcohol dehydrogenase (NAD+) activity
• GO:0004745 all-trans-retinol dehydrogenase (NAD+) activity
• GO:0008270 zinc ion binding
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0042572 retinol metabolic process
• GO:0042573 retinoic acid metabolic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:8eiy, PDBe:8eiy, PDBj:8eiy
• PDBsum: 8eiy
• PubMed: 37563323
• UniProt: P00327|ADH1E_HORSE Alcohol dehydrogenase E chain