Structure of PDB 8dh2 Chain B

Receptor sequence

>8dh2B (length=856) Species: 10760 (Escherichia phage T7) [Search protein sequence]

MNTINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEAR
FRKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRP
TAFQFLQEIKPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEAR
FGRIRDLEAKHFKKNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEA
WSSWHKEDSIHVGVRCIEMLIESTGMVSLHRQNAGVVGQDSETIELAPEY
AEAIATRAGALAGISPMFQPCVVPPKPWTGITGGGYWANGRRPLALVRTH
SKKALMRYEDVYMPEVYKAINIAQNTAWKINKKVLAVANVITKWKHCPVE
DIPAIEALTAWKRAAAAVYRKDKARKSRRISLEFMLEQANKFANHKAIWF
PYNMDWRGRVYAVSMFNPQGNDMTKGLLTLAKGKPIGKEGYYWLKIHGAN
CAGVDKVPFPERIKFIEENHENIMACAKSPLENTWWAEQDSPFCFLAFCF
EYAGVQHHGLSYNCSLPLAFDGSCSGIQHFSAMLRDEVGGRAVNLLPSET
VQDIYGIVAKKVNEILQADAINGTDNEVVTVTDENTGEISEKVKLGTKAL
AGQWLAYGVTRSVTKRSVMTLAYGSKEFGFRQQVLEDTIQPAIDSGKGLM
FTQPNQAAGYMAKLIWESVSVTVVAAVEAMNWLKSAAKLLAAEVKDKKTG
EILRKRCAVHWVTPDGFPVWQEYKKPIQTRLNLMFLGQDSEIDAHKQESG
IAPNFVHSQDGSHLRKTVVWAHEKYGIESFALIHDSFGTIPADAANLFKA
VRETMVDTYESCDVLADFYDQFADQLHESQLDKMPALPAKGNLNLRDILE
SDFAFA

3D structure

PDB

8dh2 Structural basis of transcription recognition of a hydrophobic unnatural base pair by T7 RNA polymerase.

Chain

Resolution

2.9 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	R50 R57 R298 H300 W422 R423 Y427 R632 Y639 S641 F644 Q648 Q649 E652 K713 Y739 H772 P780 H784	R50 R57 R298 H300 W406 R407 Y411 R616 Y623 S625 F628 Q632 Q633 E636 K697 Y723 H745 P753 H757
BS02	rna	B	N171 R386 K389 A390 S393 R394 Q435 N437 I810 D812	N171 R370 K373 A374 S377 R378 Q419 N421 I783 D785
BS03	dna	B	K704 E775	K688 E748
BS04	3PO	B	K472 Y571 R627 K631	K456 Y555 R611 K615

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003899	DNA-directed 5'-3' RNA polymerase activity
GO:0016779	nucleotidyltransferase activity
GO:0034062	5'-3' RNA polymerase activity

	Biological Process
GO:0006351	DNA-templated transcription
GO:0019083	viral transcription
GO:0039695	DNA-templated viral transcription

	Cellular Component
GO:0000428	DNA-directed RNA polymerase complex

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:8dh2, PDBe:8dh2, PDBj:8dh2
PDBsum	8dh2
PubMed	36635281
UniProt	P00573\|RPOL_BPT7 T7 RNA polymerase (Gene Name=1)

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