Structure of PDB 8dh1 Chain B

Receptor sequence

>8dh1B (length=855) Species: 10760 (Escherichia phage T7)

NTINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARF
RKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPT
AFQFLQEIKPEAVAYITIKTTLACLTSNTTVQAVASAIGRAIEDEARFGR
IRDLEAKHFKKNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWSS
WHKEDSIHVGVRCIEMLIESTGMVSLHRQNAGVVGQDSETIELAPEYAEA
IATRAGALAGISPMFQPCVVPPKPWTGITGGGYWANGRRPLALVRTHSKK
ALMRYEDVYMPEVYKAINIAQNTAWKINKKVLAVANVITKWKHCPVEDIP
AIEALTAWKRAAAAVYRKDKARKSRRISLEFMLEQANKFANHKAIWFPYN
MDWRGRVYAVSMFNPQGNDMTKGLLTLAKGKPIGKEGYYWLKIHGANCAG
VDKVPFPERIKFIEENHENIMACAKSPLENTWWAEQDSPFCFLAFCFEYA
GVQHHGLSYNCSLPLAFDGSCSGIQHFSAMLRDEVGGRAVNLLPSETVQD
IYGIVAKKVNEILQADAINGTDNEVVTVTDENTGEISEKVKLGTKALAGQ
WLAYGVTRSVTKRSVMTLAYGSKEFGFRQQVLEDTIQPAIDSGKGLMFTQ
PNQAAGYMAKLIWESVSVTVVAAVEAMNWLKSAAKLLAAEVKDKKTGEIL
RKRCAVHWVTPDGFPVWQEYKKPIQTRLNLMFLGQFRDSEIDAHKQESGI
APNFVHSQDGSHLRKTVVWAHEKYGIESFALIHDSFGTIPADAANLFKAV
RETMVDTYESCDVLADFYDQFADQLHESQLDKMPALPAKGNLNLRDILES
DFAFA

3D structure

• PDB: 8dh1 Structural basis of transcription recognition of a hydrophobic unnatural base pair by T7 RNA polymerase.
• Chain: B
• Resolution: 2.65 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	R50 R57 E63 R298 H300 W422 R423 Y427 R632 Y639 S641 F644 Q648 Q649 Y739 H772 P780 H784	R49 R56 E62 R295 H297 W403 R404 Y408 R613 Y620 S622 F625 Q629 Q630 Y720 H744 P752 H756
BS02	rna	B	N171 R386 K389 A390 S393 R394 R425 Q435 G436 N437 I810 D812	N168 R367 K370 A371 S374 R375 R406 Q416 G417 N418 I782 D784
BS03	S8L	B	K472 Y571 R627 K631	K453 Y552 R608 K612

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034062 5'-3' RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0019083 viral transcription
• GO:0039695 DNA-templated viral transcription

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex

External links

• PDB: RCSB:8dh1, PDBe:8dh1, PDBj:8dh1
• PDBsum: 8dh1
• PubMed: 36635281
• UniProt: P00573|RPOL_BPT7 T7 RNA polymerase (Gene Name=1)