Structure of PDB 8d9i Chain B

Receptor sequence

>8d9iB (length=1256) Species: 237368 (Candidatus Scalindua brodae)

MNITVELTFFEPYRLVEWFDWDARKKSHSAMRGQAFAQWTWKGKGRTAGK
SFITGTLVRSAVIKAVEELLSLNNGKWEGVPCCNGSFQTDESKGKKPSFL
RKRHTLQWQANNKNICDKEEACPFCILLGRFDNAGKVHERNKDYDIHFSN
FDLDHKQEKNDLRLVDIASGRILNRVDFDTGKAKDYFRTWEADYETYGTY
TGRITLRNEHAKKLLLASLGFVDKLCGALCRIEVIKKEVLSEDHNDELRK
QAEVIVEAFKQNDKLEKIRILADAIRTLRLHGEGVIEKDELPDGKEERDK
GHHLWDIKVQGTALRTKLKELWQSNKDIGWRKFTEMLGSNLYLIYKKETG
IETKEWIIVGRLKAATPFYFGVQQPSDSIPGVINEHTSFNILLDKENRYR
IPRSALRGALRRDLRTAFGSGCNVSLGGQILCNCKVCIEMRRITLKDSVS
DFSEPPEIRYRIAKNPGTATVEDGSLFDIEVGPEGLTFPFVLRYRGHKFP
EQLSSVIRYWEENDGKNGMAWLGGLDSTGKGRFALKDIKIFEWDLNQKIN
EYIKERGMRGKEKELLEMGESSLPDGLIPYKFFEERECLFPYKENLKPQW
SEVQYTIEVGSPLLTADTISALTEPGNRDAIAYKKRVYNDGNNAIEPEPR
FAVKSETHRGIFRTAVGRRTGDLGKEDHEDCTCDMCIIFGNEHESSKIRF
EDLELINGNEFEKLEKHIDHVAIDRFTGGALDKAKFDTYPLAGSPKKPLK
LKGRFWIKKGFSGDHKLLITTALSDIRDGLYPLGSKGGVGYGWVAGISID
DNVPDDFKEMINKTEAAAAAAAAAAAAAAAAAAAKNKNIYYPHYFLDSGS
KVYREKDIITHEEFTEELLSGKINCKLETLTPLIIPDTSDENGLKLQGNK
PGHKNYKFFNINGELMIPGSELRGMLRTHFEALTKSCFAIFGEDSTLSWR
RKCASKTLGGKLDKALHPCTGLSDGLCPGCHLFGTTDYKGRVKFGFAKYE
NGPEWLITRGNNPERSLTLGVLESPRPAFSIPDDESEIPGRKFYLHHNGW
RIIRQKQLEIRETVQPERNVTTEVMDKGNVFSFDVKFENLREWELGLLLQ
SLDPGKNIAHKLGKGKPYGFGSVKIKIDSLHTFKINSNNDKIKRVPQSDI
REYINKGYQKLIEWSGNNSIQKGNVLPQWHVIPHIDKLYKLLWVPFLNDS
KLEPDVRYPVLNEESKGYIEGSDYTYKKLGDKDNLPYKTRVKGLTTPWSP
WNPFQV

3D structure

• PDB: 8d9i Craspase is a CRISPR RNA-guided, RNA-activated protease.
• Chain: B
• Resolution: 3.62 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	R289 I290 K315 R318 D537 G538 L540 F541 E743 E756 K797 K799 F800 E1460 R1505	R269 I270 K295 R298 D473 G474 L476 F477 E679 E692 K733 K735 F736 E1023 R1068
BS02	rna	B	R32 Q34 F36 F52 T56 L57 R59 S60 S86 F87 D90 K93 L100 R101 K102 R103 K136 V137 E139 K142 Y144 I146 H147 F148 N150 I172 L173 N174 V176 D185 Y186 F187 G426 R467 S468 R471 R475 L490 L495 T508 L509 Y524 R525 I526 K528 V535 F541 L589 D590 S591 A680 K718 T721 R723 I725 T728 G754 D783 H784 V785 I787 G792 K799 S849 T969 S1001 E1002 R1004 G1005 R1008 T1422 K1426 V1458 E1460 S1461 P1462 R1463 Y1481 K1553	R32 Q34 F36 F52 T56 L57 R59 S60 S86 F87 D90 K93 L100 R101 K102 R103 K136 V137 E139 K142 Y144 I146 H147 F148 N150 I172 L173 N174 V176 D185 Y186 F187 G371 R403 S404 R407 R411 L426 L431 T444 L445 Y460 R461 I462 K464 V471 F477 L525 D526 S527 A616 K654 T657 R659 I661 T664 G690 D719 H720 V721 I723 G728 K735 S785 T888 S920 E921 R923 G924 R927 T985 K989 V1021 E1023 S1024 P1025 R1026 Y1044 K1116
BS03	ZN	B	H742 C745 C750	H678 C681 C686
BS04	ZN	B	C1406 C1417	C969 C980

External links

• PDB: RCSB:8d9i, PDBe:8d9i, PDBj:8d9i
• PDBsum: 8d9i
• PubMed: 36007061
• UniProt: A0A0B0EGF3