Structure of PDB 8d9f Chain B

Receptor sequence

>8d9fB (length=1242) Species: 237368 (Candidatus Scalindua brodae) [Search protein sequence]

MNITVELTFFEPYRLVEWFDWDARKKSHSAMRGQAFAQWTWKGKGRTAGK
SFITGTLVRSAVIKAVEELLSLNNGKWEGVPCCNGSFQTDESKGKKPSFL
RKRHTLQWQANNKNICDKEEACPFCILLGRFDNAGKVHERNKDYDIHFSN
FDLDHDLRLVDIASGRILNRVDFDTGKAKDYFRTWEADYETYGTYTGRIT
LRNEHAKKLLLASLGFVDKLCGALCRIEVIKSEDHNDELRKQAEVIVEAF
KQNDKLEKIRILADAIRTLRLHGEGVIEKDELPDGKEERDKGHHLWDIKV
QGTALRTKLKELWQSNKDIGWRKFTEMLGSNLYLIYKKETGGVSTRFRIL
GDTEYYSKAHDSEGSDLFIPVTPPEGIETKEWIIVGRLKAATPFYFGVQQ
PSDSIPGKEKKSEDSLVINEHTSFNILLDKENRYRIPRSALRGALRRDLR
TAFGSGCNVSLGGQILCNCKVCIEMRRITLKDSVSDFSEPPEIRYRIAKN
PGTATVEDGSLFDIEVGPEGLTFPFVLRYRGHKFPEQLSSVIRYWEENDG
KNGMAWLGGLDSTGKGRFALKDIKIFEWDLNQKINEYIKERGMRGKEKEL
LEMGESSLPDGLIPYKFFEERECLFPYKENLKPQWSEVQYTIEVGSPLLT
ADTISALTEPGNRDAIAYKKRVYNDGNNAIEPEPRFAVKSETHRGIFRTA
VGRRTGDLGKEDHEDCTCDMCIIFGNEHESSKIRFEDLELINGNEFEKLE
KHIDHVAIDRFTGGALDKAKFDTYPLAGSPKKPLKLKGRFWIKKGFSGDH
KLLITTALSDIRDGLYPLGSKGGVGYGWVAGISIDDNVPDDFKEMINKTY
VHPGHQSPKQDHKNKNIYYPHYFLDSGSKVYREKDIITHEEFTEELLSGK
INCKLETLTPLIIPDTSDENGLKLQGNKPGHKNYKFFNINGELMIPGSEL
RGMLRTHFEALTKSCFAIFGEGGKLDKALHPCTGLSDGLCPGCHLFGTTD
YKGRVKFGFAKYENGPEWLITRGNNPERSLTLGVLESPRPAFSIPDDESE
IPGRKFYLHHNGWRIIRQKQLEIRETVQPERNVTTEVMDKGNVFSFDVRF
ENLREWELGLLLQSLDPGKNIAHKLGKGKPYGFGSVKIKIDSLHTFKIIK
RVPQSDIREYINKGYQKLIEWSLPQWHVIPHIDKLYKLLWVPFLNDSKLE
PDVRYPVLNYTYKKLGDKDNLPYKTRVKGLTTPWSPWNPFQV

3D structure

PDB

8d9f Craspase is a CRISPR RNA-guided, RNA-activated protease.

Chain

Resolution

2.71 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	rna	B	E11 W18 R32 Q34 T40 K50 F52 G55 T56 L57 R59 S60 S86 F87 Q88 T89 P97 S98 F99 L100 R101 K102 R103 G135 K136 H138 Y144 H147 F148 N150 D152 I172 L173 N174 R175 V176 K182 A183 D185 Y186 F187 K224 G227 R375 D381 A388 D395 G426 R467 S468 R471 G472 R476 L490 G491 L495 T508 L509 R525 I526 K528 T534 V535 L540 F541 G588 L589 S591 A680 T721 R723 I725 R727 T728 N755 S760 D783 H784 V785 A786 I787 G793 A794 K799 F800 S849 Y917 P962 S996 E997 R999 R1003 G1416 T1417 T1418 K1421 L1454 E1455 P1457 R1458 F1461 Y1476 K1546 G1547 K1548 P1549 Y1640 Y1658	E11 W18 R32 Q34 T40 K50 F52 G55 T56 L57 R59 S60 S86 F87 Q88 T89 P97 S98 F99 L100 R101 K102 R103 G135 K136 H138 Y144 H147 F148 N150 D152 I167 L168 N169 R170 V171 K177 A178 D180 Y181 F182 K219 G222 R346 D352 A359 D366 G397 R438 S439 R442 G443 R447 L461 G462 L466 T479 L480 R496 I497 K499 T505 V506 L511 F512 G559 L560 S562 A651 T692 R694 I696 R698 T699 N726 S731 D754 H755 V756 A757 I758 G764 A765 K770 F771 S820 Y869 P914 S948 E949 R951 R955 G997 T998 T999 K1002 L1035 E1036 P1038 R1039 F1042 Y1057 K1127 G1128 K1129 P1130 Y1205 Y1212
BS02	ZN	B	C83 C116 C122 C125	C83 C116 C122 C125
BS03	ZN	B	C496 C501	C467 C472
BS04	ZN	B	H742 C745 C747 C750	H713 C716 C718 C721
BS05	ZN	B	C1013 C1401 C1409 C1412	C965 C982 C990 C993

External links

PDB	RCSB:8d9f, PDBe:8d9f, PDBj:8d9f
PDBsum	8d9f
PubMed	36007061
UniProt	A0A0B0EGF3

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