Structure of PDB 8cfh Chain B

Receptor sequence

>8cfhB (length=461) Species: 208964 (Pseudomonas aeruginosa PAO1)

GFTDYKVADITLAAWGRRELIIAESEMPALMGLRRKYAGQQPLKGAKILG
CIHMTIQTGVLIETLVALGAEVRWSSCNIFSTQDQAAAAIAAAGIPVFAW
KGETEEEYEWCIEQTILKDGQPWDANMVLDDGGDLTEILHKKYPQMLERI
HGITEETTTGVHRLLDMLKNGTLKVPAINVNDSVTKSKNDNKYGCRHSLN
DAIKRGTDHLLSGKQALVIGYGDVGKGSSQSLRQEGMIVKVAEVDPICAM
QACMDGFEVVSPYKNGINDGTEASIDAALLGKIDLIVTTTGNVNVCDANM
LKALKKRAVVCNIGHFDNEIDTAFMRKNWAWEEVKPQVHKIHRTGKDGFD
AHNDDYLILLAEGRLVNLGNATGHPSRIMDGSFANQVLAQIHLFEQKYAD
LPAAEKAKRLSVEVLPKKLDEEVALEMVKGFGGVVTQLTPKQAEYIGVSV
EGPFKPDTYRY

3D structure

• PDB: 8cfh Crystal structure of S-adenosyl-L-homocysteine hydrolase from P. aeruginosa in complex with fragment F2X-Entry C08
• Chain: B
• Resolution: 1.6 Å

Enzymatic activity

• Enzyme Commision number: 3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	B	T165 T166 T167 N199 I227 G230 D231 V232 E251 V252 T298 N300 V303 I321 G322 H323 N375 H382	T157 T158 T159 N191 I219 G222 D223 V224 E243 V244 T290 N292 V295 I313 G314 H315 N367 H374
BS02	ADE	B	H61 T63 H382 M387	H53 T55 H374 M379
BS03	R8V	B	T466 Y467 R468	T458 Y459 R460
BS04	R8V	B	D465 T466 R468	D457 T458 R460
BS05	NAD	B	K463 Y467	K455 Y459
BS06	R8V	B	N187 D190 V422	N179 D182 V414

Gene Ontology

Molecular Function

• GO:0004013 adenosylhomocysteinase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0033353 S-adenosylmethionine cycle
• GO:0071269 L-homocysteine biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:8cfh, PDBe:8cfh, PDBj:8cfh
• PDBsum: 8cfh
• UniProt: Q9I685|SAHH_PSEAE Adenosylhomocysteinase (Gene Name=ahcY)