Structure of PDB 8c8h Chain B

Receptor sequence

>8c8hB (length=1129) Species: 502057 (Vaccinia virus GLV-1h68)

EMDQRLGYKFLVPDPKAGVFYRPLHFQYVSYSNFILHRLHEILTVKRPLL
SFKNNTERIMIEISNVKVTPPDYSPIIASIKGKSYDALATFTVNIFKEVM
TKEGISITKISSYEGKDSHLIKIPLLIGYGNKNPLDTAKYLVPNVIGGVF
INKQSVEKVGINLVEKITTWPKFRVVKPNSFTFSFSSVSPPNVLPTRYRH
YKISLDISQLEALNISSTKTFITVNIVLLSQYLSRVSLEFIRRSLSYDMP
PEVVYLVNAIIDSAKRITESITDFNIDTYINDLVEAEHIKQKSQLTINEF
KYEMLHNFLPHMNYTPDQLKGFYMISLLRKFLYCIFHTSRYPDRDSMVCH
RILTYGKYFETLAHDELENYIGNIRNDIMNNHKNRGTYAVNIHVLTTPGL
NHAFSSLLSGKFKKSDGSYRTHPHYSWMQNISIPRSVGFYPVRKYHPSQY
LYFCSSDVPERGPQVGLVSQLSVLSSITNILTSEYLDLEKKICEYIRSYY
KDDISYFETGFPITIENALVASLNPNMICDFVTDFRRRKRMGFFGNLEVG
ITLVRDHMNEIRINIGAGRLVRPFLVVDNGELMMDVCPELESRLDDMTFS
DIQKEFPHVIEMVDIEQFTFSNVCESVQKFRMMSKDERKQYDLCDFPAEF
RDGYVASSLVGINHNSGPRAILGCAQAKQAISCLSSDIRNKIDNGIHLMY
PERPIVISKALETSKIAANCFGQHVTIALMSYKGINQEDGIIIKKQFIQR
GGLDIVTAKKHQVEIPLENFNNKERDRSNAYSKLESNGLVRLNAFLESGD
AMARNISSVSEKYTDMYKSRVERVQVELTDKVKVRVLTMKERRPILGDKF
TTRTSQKGTVAYVADETELPYDENGITPDVIINSTSIFSRKTISMLIEVI
LTAAYSAKPYNNKGENRPVCFPSSNETSIDTYMQFAKQCYEHSNPKLSDE
ELSDKIFCEKILYDPETDKPYASKVFFGPIYYLRLRHLTQDKATVRCRGK
KTKLIRQANEGRKRGGGIKFGEMERDCLIAHGAANTITEVLKDSEEDYQD
VYVCENCGDIAAQIKGINTCLRCSKLNLSPLLTKIDTTHVSKVFLTQMNA
RGVKVKLDFERRPPSFYKPLDKVDLKPSF

3D structure

• PDB: 8c8h Structural Basis of Poxvirus Transcription: Vaccinia RNA Polymerase Complexes.
• Chain: B
• Resolution: 3.84 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	B	C1087 C1106	C1054 C1073

Gene Ontology

Molecular Function

• GO:0001054 RNA polymerase I activity
• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0032549 ribonucleoside binding
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0006360 transcription by RNA polymerase I

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex
• GO:0044423 virion component

External links

• PDB: RCSB:8c8h, PDBe:8c8h, PDBj:8c8h
• PDBsum: 8c8h
• UniProt: Q76ZP7|RP132_VACCW DNA-directed RNA polymerase 133 kDa polypeptide (Gene Name=OPG151)