Structure of PDB 8bf5 Chain B

Receptor sequence

>8bf5B (length=726) Species: 1601067 (Influenza B virus (B/Memphis/13/2003))

GMNINPYFLFIDVPIQAAISTTFPYTGVPPYSHGTGTGYTIDTVIRTHEY
SNKGKQYISDVTGCTMVDPTNGPLPEDNEPSAYAQLDCVLEALDRMDEEH
PGLFQAASQNAMETLMVTTVDKLTQGRQTFDWTVCRNQPAATALNTTITS
FRLNDLNGADKGGLIPFCQDIIDSLDRPEMTFFSVKNIKKKLFLIKRIPM
KVKDKITKVEYIKRALSLNTMTKDAERGKLKRRAIATAGIQIRGFVLVVE
NLAKNICENLEQSGLPVGGNEKKAKLSNAVAKMLSNCPPGGISMTVTGDN
TKWNECLNPRIFLAMTERITRDSPIWFRDFCSIAPVLFSNKIARLGKGFM
ITSKTKRLKAQIPCPDLFSIPLERYNEETRAKLKKLKPFFNEEGTASLSP
GMMMGMFNMLSTVLGVAALGIKNIGNKEYLWDGLQSSDDFALFVNAKDEE
TCMEGINDFYRTCKLLGINMSKKKSYCNETGMFEFTSMFYRDGFVSNFAM
ELPSFGVAGVNESADMAIGMTIIKNNMINNGMGPATAQTAIQLFIADYRY
TYKCHRGDSKVEGKRMKIIKELWENTKGRDGLLVADGGPNIYNLRNLHIP
EIVLKYNLMDPEYKGRLLHPQNPFVGHLSKMDYDAVSGTHSWRTKRNRSI
LNTDQRNMILEEQCYAKCCNLFEACFNSASYRKPVGQHSMLEAMAHRLRM
DARLDYESGRMSKDDFEKAMAHLGEI

3D structure

• PDB: 8bf5 Direct observation of backtracking by influenza A and B polymerases upon consecutive incorporation of the nucleoside analog T1106.
• Chain: B
• Resolution: 2.96 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	H32 G33 T34 Y38 L200 K365 L674 N675	H33 G34 T35 Y39 L194 K359 L651 N652
BS02	rna	B	R126 Q127 N136 M227 K229 D230 I241 A242 R249 P272 V273 G274 G275 M412 N414 M415 I524 T527 N531	R127 Q128 N137 M221 K223 D224 I235 A236 R243 P266 V267 G268 G269 M406 N408 M409 I518 T521 N525
BS03	rna	B	Y24 Q124 R126 D444 T492 S493 M506 P509 S510	Y25 Q125 R127 D438 T486 S487 M500 P503 S504
BS04	G2P	B	K229 R239 T307 K308 N310 D444 K480	K223 R233 T301 K302 N304 D438 K474
BS05	MG	B	D305 D445	D299 D439

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019083 viral transcription
• GO:0039523 symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity
• GO:0039694 viral RNA genome replication

Cellular Component

• GO:0030430 host cell cytoplasm
• GO:0042025 host cell nucleus

External links

• PDB: RCSB:8bf5, PDBe:8bf5, PDBj:8bf5
• PDBsum: 8bf5
• PubMed: 36596301
• UniProt: Q5V8Y6