Structure of PDB 8bek Chain B

Receptor sequence

>8bekB (length=732) Species: 1318616 (Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))

MDVNPTLLFLKVPVQNAISTTFPYTGDPPYSHGTGTGYTMDTVNRTHKYS
EKGKWTTNTETGAPQLNPIDGPLPEDNEPSGYAQTDCVLEAMAFLEESHP
GIFENSCLETMEIVQQTRVDKLTQGRQTYDWTLNRNQPAATALANTIEVF
RSNGLTANESGRLIDFLKDVMDSMDKEEMEITTHFQIGKKKQRLNKRSYL
IRALTLNTMTKDAERGKLKRRAIATPGMQIRGFVYFVEALARSICEKLEQ
SGLPVGGNEKKAKLANVVRKMMTNSQDTELSFTITGDNTKWNENQNPRMF
LAMITYITRNQPEWFRNVLSIAPIMFSNKMARLGKGYMFESKSMKLRTQV
PAEMLANIDLKYFNKSTREKIEKIRPLLIDGTASLSPGMMMGMFNMLSTV
LGVSILNLGQKKYTKTTYWWDGLQSSDDFALIVNAPNHEGIQAGVDRFYR
TCKLVGINMSKKKSYINRTGTFEFTSFFYRYGFVANFSMELPSFGVSGIN
ESADMSVGVTVIKNNMINNDLGPATAQMALQLFIKDYRYTYRCHRGDTQI
QTRRAFELKKLWEQTRSKAGLLVSDGGPNLYNIRNLHIPEVCLKWELMDE
DYQGRLCNPMNPFVSHKEIDSVNNAVVMPSMEYDAVATTHSWIPKRNRSI
LNTSQRGILEDEQMYQKCCNLFEKFFPSSSYRRPVGISSMVEAMVSRARI
DARIDFESGRIKKEEFAEIMKICSTIEELRRQ

3D structure

• PDB: 8bek Direct observation of backtracking by influenza A and B polymerases upon consecutive incorporation of the nucleoside analog T1106.
• Chain: B
• Resolution: 2.86 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	R45 Q124 K229 K235 R239 E264 T307 K308 N310 G410 D445 M507 E656	R45 Q124 K211 K217 R221 E246 T289 K290 N292 G392 D427 M489 E632
BS02	rna	B	R126 Q127 I241 T243 R260 P272 G274 G410 M655 E656 Y657 N671 R672 N676	R126 Q127 I223 T225 R242 P254 G256 G392 M631 E632 Y633 N647 R648 N652
BS03	rna	B	H32 T34 M356 R365	H32 T34 M338 R347
BS04	MG	B	G304 D305 D446	G286 D287 D428

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019083 viral transcription
• GO:0039523 symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity
• GO:0039694 viral RNA genome replication

Cellular Component

• GO:0005737 cytoplasm
• GO:0030430 host cell cytoplasm
• GO:0042025 host cell nucleus

External links

• PDB: RCSB:8bek, PDBe:8bek, PDBj:8bek
• PDBsum: 8bek
• PubMed: 36596301
• UniProt: M9TLW3