Structure of PDB 8bcb Chain B

Receptor sequence

>8bcbB (length=1721) Species: 9606 (Homo sapiens)

ALAPRQVLDLEDLVFTQGSHFMANKRCQLPDGSFRRQRKGYEEVHVPALK
PKPFGSEEQLLPVEKLPKYAQAGFEGFKTLNRIQSKLYRAALETDENLLL
CAPTGAGKTNVALMCMLREIGKHINMDGTINVDDFKIIYIAPMRSLVQEM
VGSFGKRLATYGITVAELTGDHQLCKEEISATQIIVCTPEKWDIITRKGG
ERTYTQLVRLIILDEIHLLHDDRGPVLEALVARAIRNIEMTQEDVRLIGL
SATLPNYEDVATFLRVDPAKGLFYFDNSFRPVPLEQTYVGITEKKAIKRF
QIMNEIVYEKIMEHAGKNQVLVFVHSRKETGKTARAIRDMCLEKDTLGLF
LREGSASTEVLRTEAEQCKNLELKDLLPYGFAIHHAGMTRVDRTLVEDLF
ADKHIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPEKGRWTELGALDILQ
MLGRAGRPQYDTKGEGILITSHGELQYYLSLLNQQLPIESQMVSKLPDML
NAEIVLGNVQNAKDAVNWLGYAYLYIRMLRSPTLYGISHDDLKGDPLLDQ
RRLDLVHTAALMLDKNNLVKYDKKTGNFQVTELGRIASHYYITNDTVQTY
NQLLKPTLSEIELFRVFSLSSEFKNITVREEEKLELQKLLERVPIPVKES
IEEPSAKINVLLQAFISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLN
RGWAQLTDKTLNLCKMIDKRMWQSMCPLRQFRKLPEEVVKKIEKKNFPFE
RLYDLNHNEIGELIRMPKMGKTIHKYVHLFPKLELSVHLQPITRSTLKVE
LTITPDFQWDEKVHGSSEAFWILVEDVDSEVILHHEYFLLKAKYAQDEHL
ITFFVPVFEPLPPQYFIRVVSDRWLSCETQLPVSFRHLILPEKYPPPTEL
LDLQPLPVSALRNSAFESLYQDKFPFFNPIQTQVFNTVYNSDDNVFVGAP
TGSGKTICAEFAILRMLLQSSEGRCVYITPMEALAEQVYMDWYEKFQDRL
NKKVVLLTGETSTDLKLLGKGNIIISTPEKWDILSRRWKQRKNVQNINLF
VVDEVHLIGGENGPVLEVICSRMRYISSQIERPIRIVALSSSLSNAKDVA
HWLGCSATSTFNFHPNVRPVPLELHIQGFNISHTQTRLLSMAKPVYHAIT
KHSPKKPVIVFVPSRKQTRLTAIDILTTCAADIQRQRFLHCTEKDLIPYL
EKLSDSTLKETLLNGVGYLHEGLSPMERRLVEQLFSSGAIQVVVASRSLC
WGMNVAAHLVIIMDTQYYNGKIHAYVDYPIYDVLQMVGHANRPLQDDEGR
CVIMCQGSKKDFFKKFLYEPLPVESHLDHCMHDHFNAEIVTKTIENKQDA
VDYLTWTFLYRRMTQNPNYYNLQGISHRHLSDHLSELVEQTLSDLEQSKC
ISIEDEMDVAPLNLGMIAAYYYINYTTIELFSMSLNAKTKVRGLIEIISN
AAEYENIPIRHHEDNLLRQLAQKVPHKLNNPKFNDPHVKTNLLLQAHLSR
MQLSAELQSDTEEILSKAIRLIQACVDVLSSNGWLSPALAAMELAQMVTQ
AMWSKDSYLKQLPHFTSEHIKRCTDKGVESVFDIMEMEDEERNALLQLTD
SQIADVARFCNRYPNIELSYEVVDKDSIRSGGPVVVLVQLEREEEVTGPV
IAPLFPQKREEGWWVVIGDAKSNSLISIKRLTLQQKAKVKLDFVAPATGA
HNYTLYFMSDAYMGCDQEYKF

3D structure

• PDB: 8bcb Conformation-dependent ligand hot spots in the spliceosomal RNA helicase BRR2.
• Chain: B
• Resolution: 2.38 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SAN	B	N1101 N1531 I1532 S1533 Q1707 G1708	N700 N1130 I1131 S1132 Q1306 G1307
BS02	SAN	B	W593 T606 N638	W192 T205 N237

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0005524 ATP binding

External links

• PDB: RCSB:8bcb, PDBe:8bcb, PDBj:8bcb
• PDBsum: 8bcb
• PubMed: 36974964
• UniProt: O75643|U520_HUMAN U5 small nuclear ribonucleoprotein 200 kDa helicase (Gene Name=SNRNP200)