Structure of PDB 8b37 Chain B

Receptor sequence

>8b37B (length=681) Species: 13773 (Pyrobaculum aerophilum)

YALLGVILGISGVIYAVYLAVWVLRNEKMRFISQAIATGARAYLFRQYRT
LAVLLVILAVLILVAIDGLTALAFIVGALGSMLAGYLGMYVTTRSASRVA
QAAATGGMGKALLVSWRAGAVMGLSLASIALLLISGFYLVFRSVLPDDWA
VPLVALGFGASLVTLFMRVGGGIYTKAADLGADLVGKVEAGIPEDDPRNP
GVIADNVGDNVGDVAGMAADVYESYIVTVTAAIFLAAILGLPTQFIEAII
LFAALALVATFAGVNLGVKHPLSSISLAIYATIGLSVVLFFIGAFTLGLD
STKALALAATTSLGAVIAPLIVKITDYYTSYNYGPVRKIAEQAKISPATV
IITGYGVGLMSAIPVIAVIVAVLGISYMIGYYTVFGELSKYLAGIFGTAM
ASVGLLVVAGIIITADSYGPVSDNAGGVVEMAGLPDEVREITDVLDSVGN
TTKATTKGYAIASAALAALVLFIALIFEIVYSASKDMISESLSGLQLINA
NVLIGAFLGVALVYFFSSRTLEAVGRTAMEIVEEIRRQFREKPGILEPDY
ARVVDIATRRALGEFLIPGLAAIVLPLITGLLLGWNALAGLIMGAIVAGV
PRALLMANAGGAWDNAKKYIEIQGLKKTEMHKAAVIGDTVGDPMKDTVGP
SLNPLIKVLNTLSVVFTYVIVSTNIALGIWP

3D structure

• PDB: 8b37 Functional and structural asymmetry suggest a unifying principle for catalysis in membrane-bound pyrophosphatases.
• Chain: B
• Resolution: 3.84 Å

Enzymatic activity

• Enzyme Commision number: 7.1.3.1: H(+)-exporting diphosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	B	D194 D198 E209	D179 D183 E194
BS02	MG	B	D194 D676	D179 D642
BS03	MG	B	D224 D446	D209 D423
BS04	MG	B	N473 D648	N450 D614
BS05	2PN	B	D194 D446 K476 D648 K679	D179 D423 K453 D614 K645

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0004427 inorganic diphosphate phosphatase activity
• GO:0009678 diphosphate hydrolysis-driven proton transmembrane transporter activity
• GO:0046872 metal ion binding

Biological Process

• GO:1902600 proton transmembrane transport

Cellular Component

• GO:0005886 plasma membrane
• GO:0016020 membrane

External links

• PDB: RCSB:8b37, PDBe:8b37, PDBj:8b37
• PDBsum: 8b37
• PubMed: 38182815
• UniProt: Q8ZWI8|HPPA_PYRAE K(+)-insensitive pyrophosphate-energized proton pump (Gene Name=hppA)