Structure of PDB 8b1t Chain B

Receptor sequence
>8b1tB (length=1141) Species: 562 (Escherichia coli) [Search protein sequence]
AETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGLGGSAAFPRPL
TVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLEEI
DDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIE
DESLLRYQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGE
APVIKAPPPDDETLASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDR
RKFNRSNQAKWIDKISAWAEEETNSYQLPESLEKFSQRFLEDRTKAGGET
PRHPLFEAIDQLLAEPLSIRDLVITRALAEIRETVAREKRRRGELGFDDM
LSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQQYRIFRRIWHHQ
PETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSAPGMV
NSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLM
EGESCGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDI
SVLVRSRQEAAQVRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVM
TPERENTLRSALATSMMGLNALDIETLNNDEHAWDVVVEEFDGYRQIWRK
RGVMPMLRALMSARNIAENLLATAGGERRLTDILHISELLQEAGTQLESE
HALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVTIHKSKGLEYPLVWL
PFITNFRVQEQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAEDLRLLYV
ALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLR
TCIEALCDDDIAWQTAQTGDNQPWQVNDVSTAELNAKTLQRLPGDNWRVT
SYSGLQQTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNWVREKLELGGFE
SQWEPVLTEWITAVLQAPLNETGVSLSQLSARNKQVEMEFYLPISEPLIA
SQLDTLIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLAYKS
NWLGEDSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYEH
HFGGVIYLFLRGVDKEHPQQGIYTTRPNAGLIALMDEMFAG
3D structure
PDB8b1t Structures of RecBCD in complex with phage-encoded inhibitor proteins reveal distinctive strategies for evasion of a bacterial immunity hub.
ChainB
Resolution3.4 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.11.5: exodeoxyribonuclease V.
5.6.2.4: DNA 3'-5' helicase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B R254 R255 K256 N258 W265 K288 R297 F422 R423 V511 R561 H742 K743 R761 R822 R823 R824 G825 R250 R251 K252 N254 W261 K284 R293 F418 R419 V507 R557 H738 K739 R757 R818 R819 R820 G821
BS02 ANP B G26 G28 K29 T30 F31 W447 R448 R808 G22 G24 K25 T26 F27 W443 R444 R804
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0003677 DNA binding
GO:0003678 DNA helicase activity
GO:0004386 helicase activity
GO:0004520 DNA endonuclease activity
GO:0004527 exonuclease activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008094 ATP-dependent activity, acting on DNA
GO:0008854 exodeoxyribonuclease V activity
GO:0015616 DNA translocase activity
GO:0016787 hydrolase activity
GO:0016853 isomerase activity
GO:0016887 ATP hydrolysis activity
GO:0043138 3'-5' DNA helicase activity
GO:0046872 metal ion binding
Biological Process
GO:0000724 double-strand break repair via homologous recombination
GO:0000725 recombinational repair
GO:0006281 DNA repair
GO:0006310 DNA recombination
GO:0009314 response to radiation
GO:0032508 DNA duplex unwinding
GO:0044355 clearance of foreign intracellular DNA
Cellular Component
GO:0005829 cytosol
GO:0009338 exodeoxyribonuclease V complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8b1t, PDBe:8b1t, PDBj:8b1t
PDBsum8b1t
PubMed36533901
UniProtP08394|RECB_ECOLI RecBCD enzyme subunit RecB (Gene Name=recB)

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