Structure of PDB 8a8w Chain B

Receptor sequence

>8a8wB (length=568) Species: 1773 (Mycobacterium tuberculosis)

SLVLDQFGRNLTAAAMEGKLDPVIGREKEIERVMQVLSRRTKNNPVLIGE
PGVGKTAVVEGLAQAIVHGEVPETLKDKQLYTLDLGSLVAGSRYRGDFEE
RLKKVLKEINTRGDIILFIDELHTLVGAGAAEGAIDAASILKPKLARGEL
QTIGATTLDEYRKYIEKDAALERRFQPVQVGEPTVEHTIEILKGLRDRYE
AHHRVSITDAAMVAAATLADRYINDRFLPDKAIDLIDEAGARMRIRRMVA
EVDDEQIAEVLGNWTGIPVFKLTEAETTRLLRMEEELHKRIIGQEDAVKA
VSKAIRRTRAGLKDPKRPSGSFIFAGPSGVGKTELSKALANFLFGDDDAL
IQIDMGEFHDRFTASRLFGAPPGYVGYEEGGQLTEKVRRKPFSVVLFDEI
EKAHQEIYNSLLQVLEDGRLTDGQGRTVDFKNTVLIFTSNLYERMKQKVN
DELKKHFRPEFLNRIDDIIVFHQLTREEIIRMVDLMISRVAGQLKSKDMA
LVLTDAAKALLAKRGFDPVLGARPLRRTIQREIEDQLSEKILFEEVGPGQ
VVTVDVDNWDEDAVFTFT

3D structure

• PDB: 8a8w Structure of the drug target ClpC1 unfoldase in action provides insights on antibiotic mechanism of action.
• Chain: B
• Resolution: 4.29 Å

Enzymatic activity

• Enzyme Commision number: 3.4.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	B	Y261 R262 A298 E299 G600 Y601 V602	Y94 R95 A131 E132 G373 Y374 V375
BS02	ADP	B	D188 P189 V190 I191 P218 G219 V220 G221 K222 T223 I358 L362 D397	D21 P22 V23 I24 P51 G52 V53 G54 K55 T56 I191 L195 D230
BS03	ADP	B	R517 I518 G556 V557 G558 K559 T560 E561 M730 R771 R774	R290 I291 G329 V330 G331 K332 T333 E334 M482 R523 R526
BS04	ADP	B	R314 R340	R147 R173

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016887 ATP hydrolysis activity
• GO:0042803 protein homodimerization activity
• GO:0044183 protein folding chaperone

Biological Process

• GO:0006457 protein folding

Cellular Component

• GO:0005829 cytosol
• GO:0005886 plasma membrane
• GO:0009274 peptidoglycan-based cell wall

External links

• PDB: RCSB:8a8w, PDBe:8a8w, PDBj:8a8w
• PDBsum: 8a8w
• PubMed: 36208775
• UniProt: P9WPC9|CLPC1_MYCTU ATP-dependent Clp protease ATP-binding subunit ClpC1 (Gene Name=clpC1)