Structure of PDB 8a7f Chain B

Receptor sequence
>8a7fB (length=365) Species: 160488,224911 [Search protein sequence]
GSHMINAKLLQLMVEHSNDGIVVAEQEGNESILIYVNPAFERLTGYCADD
ILYQDCRFLQGEDHDQPGRAIIREAIREGRPCCQVLRNYRKDGSLFWNEL
SITPVHNEADQLTYYIGIQRDVTEHQQTQARLQELQSELVHVSRLSAMGE
MASALAHELNQPLAAISNYMKGSRRLLAGSSDPNTPKVESALDRAAEQAL
RAGQIIRRLRDFVARGESEKRVESLSKLIEEAGALGLAGAREQNVQLRFS
LDPGADLVLADRVQIQQVLVNLFRNALEAMAQSQRRELVVTNTPAADDMI
EVEVSDTGSGFQDDVIPNLFQTFFTTKDTGMGVGLSISRSIIEAHGGRMW
AESNASGGATFRFTL
3D structure
PDB8a7f Crystal structure of a chimeric LOV-Histidine kinase SB2F1-I66R mutant (asymmetrical variant, trigonal form with long c axis)
ChainB
Resolution2.71 Å
3D
structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Enzyme Commision number 2.7.13.3: histidine kinase.
Interaction with ligand
Site
#
Ligand Ligand
chain
Binding residues on receptor
(original residue number in PDB)
Binding residues on receptor
(residue number reindexed from 1)
Binding affinity
BS01 ATP B N268 N272 A273 F308 L316 T322 T323 K324 M328 G329 V330 G331 L332 F358 N271 N275 A276 F311 L319 T325 T326 K327 M331 G332 V333 G334 L335 F361
BS02 FMN B V19 D52 C53 R54 Q57 R66 I69 R70 N85 I99 Y112 G114 Q116 V22 D55 C56 R57 Q60 R69 I72 R73 N88 I102 Y115 G117 Q119
Gene Ontology
Molecular Function
GO:0000155 phosphorelay sensor kinase activity
GO:0016772 transferase activity, transferring phosphorus-containing groups
Biological Process
GO:0007165 signal transduction
GO:0016310 phosphorylation

View graph for
Molecular Function

View graph for
Biological Process
External links
PDB RCSB:8a7f, PDBe:8a7f, PDBj:8a7f
PDBsum8a7f
PubMed
UniProtP23222|FIXL_BRADU Sensor protein FixL (Gene Name=fixL);
Q88JB0

[Back to BioLiP]