Structure of PDB 8a1x Chain B

Receptor sequence

>8a1xB (length=396) Species: 666 (Vibrio cholerae)

KHEKWFALYEAAATLFYTPGLVTKRSSHVRDSVDLKRIMIMVWLAVFPAM
FWGMYNAGGQAIAALNHLYSGDQLAAIVAGNWHYWLTEMLGGTMSSDAGW
GSKMLLGATYFLPIYATVFIVGGFWEVLFCMVRKHEVNEGFFVTSILFAL
IVPPTLPLWQAALGITFGVVVAKEVFGGTGRNFLNPALAGRAFLFFAYPA
QISGDLVWTAADGYSGATALSQWAQGGAGALINNATGQTITWMDAFIGNI
PGSIGEVSTLALMIGAAFIVYMGIASWRIIGGVMIGMILLSTLFNVIGSD
TNAMFNMPWHWHLVLGGFAFGMFFMATDPVSASFTNSGKWAYGILIGVMC
VLIRVVNPAYPEGMMLAILFANLFAPLFDHVVVERNIKRRLARYGK

3D structure

• PDB: 8a1x Conformational coupling of redox-driven Na + -translocation in Vibrio cholerae NADH:quinone oxidoreductase.
• Chain: B
• Resolution: 3.2 Å

Enzymatic activity

• Enzyme Commision number: 7.2.1.1: NADH:ubiquinone reductase (Na(+)-transporting).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FMN	B	R209 F213 W226 T236 S239 E274 G334 G335 P379 E380 M383	R191 F195 W208 T218 S221 E256 G316 G317 P361 E362 M365
BS02	RBF	B	M57 V60 V161 T162 L165 T197 N200 N203 P204 A205 I292 F342 M343 D346 P347 V348	M39 V42 V143 T144 L147 T179 N182 N185 P186 A187 I274 F324 M325 D328 P329 V330
BS03	FMN	B	F213 P217 D223 A377	F195 P199 D205 A359

Gene Ontology

Molecular Function

• GO:0010181 FMN binding
• GO:0016655 oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
• GO:1902444 riboflavin binding

Biological Process

• GO:0006814 sodium ion transport
• GO:0022904 respiratory electron transport chain
• GO:0055085 transmembrane transport

Cellular Component

• GO:0005886 plasma membrane
• GO:0016020 membrane

External links

• PDB: RCSB:8a1x, PDBe:8a1x, PDBj:8a1x
• PDBsum: 8a1x
• PubMed: 37710014
• UniProt: A5F5X0|NQRB_VIBC3 Na(+)-translocating NADH-quinone reductase subunit B (Gene Name=nqrB)