Structure of PDB 8a1u Chain B

Receptor sequence

>8a1uB (length=395) Species: 666 (Vibrio cholerae)

HEKWFALYEAAATLFYTPGLVTKRSSHVRDSVDLKRIMIMVWLAVFPAMF
WGMYNAGGQAIAALNHLYSGDQLAAIVAGNWHYWLTEMLGGTMSSDAGWG
SKMLLGATYFLPIYATVFIVGGFWEVLFCMVRKHEVNEGFFVTSILFALI
VPPTLPLWQAALGITFGVVVAKEVFGGTGRNFLNPALAGRAFLFFAYPAQ
ISGDLVWTAADGYSGATALSQWAQGGAGALINNATGQTITWMDAFIGNIP
GSIGEVSTLALMIGAAFIVYMGIASWRIIGGVMIGMILLSTLFNVIGSDT
NAMFNMPWHWHLVLGGFAFGMFFMATDPVSASFTNSGKWAYGILIGVMCV
LIRVVNPAYPEGMMLAILFANLFAPLFDHVVVERNIKRRLARYGK

3D structure

• PDB: 8a1u Conformational coupling of redox-driven Na + -translocation in Vibrio cholerae NADH:quinone oxidoreductase.
• Chain: B
• Resolution: 2.86 Å

Enzymatic activity

• Enzyme Commision number: 7.2.1.1: NADH:ubiquinone reductase (Na(+)-transporting).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FMN	B	I169 R209 F213 W226 T236 L238 S239 E274 G334 G335 P379 E380 M383	I150 R190 F194 W207 T217 L219 S220 E255 G315 G316 P360 E361 M364
BS02	RBF	B	M57 V60 V161 T162 L165 T197 G198 N200 N203 P204 A205 I292 F342 M343 D346 P347 V348	M38 V41 V142 T143 L146 T178 G179 N181 N184 P185 A186 I273 F323 M324 D327 P328 V329
BS03	UQ2	B	L26 G141 F142 V145 V155 N156 E157 F160	L7 G122 F123 V126 V136 N137 E138 F141
BS04	NA	B	A263 I268 V275 V332	A244 I249 V256 V313
BS05	NA	B	I371 R372 N375 P376 Y378 P379	I352 R353 N356 P357 Y359 P360
BS06	FMN	B	F213 P217 A377	F194 P198 A358

Gene Ontology

Molecular Function

• GO:0010181 FMN binding
• GO:0016655 oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor

Biological Process

• GO:0006814 sodium ion transport
• GO:0022904 respiratory electron transport chain
• GO:0055085 transmembrane transport

Cellular Component

• GO:0005886 plasma membrane
• GO:0016020 membrane

External links

• PDB: RCSB:8a1u, PDBe:8a1u, PDBj:8a1u
• PDBsum: 8a1u
• PubMed: 37710014
• UniProt: Q9KPS2|NQRB_VIBCH Na(+)-translocating NADH-quinone reductase subunit B (Gene Name=nqrB)