Structure of PDB 8a1t Chain B

Receptor sequence

>8a1tB (length=392) Species: 666 (Vibrio cholerae)

WFALYEAAATLFYTPGLVTKRSSHVRDSVDLKRIMIMVWLAVFPAMFWGM
YNAGGQAIAALNHLYSGDQLAAIVAGNWHYWLTEMLGGTMSSDAGWGSKM
LLGATYFLPIYATVFIVGGFWEVLFCMVRKHEVNEGFFVTSILFALIVPP
TLPLWQAALGITFGVVVAKEVFGGTGRNFLNPALAGRAFLFFAYPAQISG
DLVWTAADGYSGATALSQWAQGGAGALINNATGQTITWMDAFIGNIPGSI
GEVSTLALMIGAAFIVYMGIASWRIIGGVMIGMILLSTLFNVIGSDTNAM
FNMPWHWHLVLGGFAFGMFFMATDPVSASFTNSGKWAYGILIGVMCVLIR
VVNPAYPEGMMLAILFANLFAPLFDHVVVERNIKRRLARYGK

3D structure

• PDB: 8a1t Conformational coupling of redox-driven Na + -translocation in Vibrio cholerae NADH:quinone oxidoreductase.
• Chain: B
• Resolution: 3.37 Å

Enzymatic activity

• Enzyme Commision number: 7.2.1.1: NADH:ubiquinone reductase (Na(+)-transporting).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FMN	B	R209 F213 W226 T236 L238 S239 E274 G334 G335 P379 E380 G381 M383	R187 F191 W204 T214 L216 S217 E252 G312 G313 P357 E358 G359 M361
BS02	RBF	B	M57 V161 T162 L165 T197 G198 N200 N203 P204 A205 F342 M343 D346 P347 V348	M35 V139 T140 L143 T175 G176 N178 N181 P182 A183 F320 M321 D324 P325 V326
BS03	NA	B	A263 V275 T277 V332	A241 V253 T255 V310
BS04	FMN	B	F213 P217 D223 A377	F191 P195 D201 A355

Gene Ontology

Molecular Function

• GO:0010181 FMN binding
• GO:0016655 oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
• GO:1902444 riboflavin binding

Biological Process

• GO:0006814 sodium ion transport
• GO:0022904 respiratory electron transport chain
• GO:0055085 transmembrane transport

Cellular Component

• GO:0005886 plasma membrane
• GO:0016020 membrane

External links

• PDB: RCSB:8a1t, PDBe:8a1t, PDBj:8a1t
• PDBsum: 8a1t
• PubMed: 37710014
• UniProt: A5F5X0|NQRB_VIBC3 Na(+)-translocating NADH-quinone reductase subunit B (Gene Name=nqrB)