Structure of PDB 7z1o Chain B

Receptor sequence

>7z1oB (length=1101) Species: 580240 (Saccharomyces cerevisiae W303)

INTAQDKWHLLPAFLKVKGLVKQHLDSFNYFVDTDLKKIIKANQLILSDV
DPEFYLKYVDIRVGKKSSSSTKDYLTPPHECRLRDMTYSAPIYVDIEYTR
GRNIIMHKDVEIGRMPIMLRSNKCILYDADESKMAKLNECPLDPGGYFIV
NGTEKVILVQEQLSKNRIIVEADEKKGIVQASVTSSTHERKSKTYVITKN
GKIYLKHNSIAEEIPIAIVLKACGILSDLEIMQLVCGNDSSYQDIFAVNL
EESSKLDIYTQQQALEYIGAKVKTMRRQKLTILQEGIEAIATTVIAHLTV
EALDFREKALYIAMMTRRVVMAMYNPKMIDDRDYVGNKRLELAGQLISLL
FEDLFKKFNNDFKLSIDKVLKKPNRAMEYDALLSINVHSNNITSGLNRAI
STGNWSLKRFKMERAGVTHVLSRLSYISALGMMTRISSQFEKSRKVSGPR
ALQPSQFGMLCTADTPEGEACGLVKNLALMTHITTDDEEEPIKKLCYVLG
VEDITLIDSASLHLNYGVYLNGTLIGSIRFPTKFVTQFRHLRRTGKVSEF
ISIYSNSHQMAVHIATDGGRICRPLIIVSDGQSRVKDIHLRKLLDGELDF
DDFLKLGLVEYLDVNEENDSYIALYEKDIVPSMTHLEIEPFTILGAVAGL
IPYPHHNQSPRNTYQCAMGKQAIGAIAYNQFKRIDTLLYLMTYPQQPMVK
TKTIELIDYDKLPAGQNATVAVMSYSGYDIEDALVLNKSSIDRGFGRCET
RRKTTTVLKRYANHTQDIIGGMRVDENGDPIWQHQSLGPDGLGEVGMKVQ
SGQIYINKSVPTNSQTQYREAPVIYRGPEPSHIDQVMMSVSDNDQALIKV
LLRQNRRPELGDKFSSRHGQKGVCGIIVKQEDMPFNDQGIVPDIIMNPHG
FPSRMTVGKMIELISGKAGVLNGTLEYGTCFGGSKLEDMSKILVDQGFNY
SGKDMLYSGITGECLQAYIFFGPIYYQKLKHMVLDKMHARARGPRAVLTR
QPTEGRSRDGGLRLGEMERDCVIAYGASQLLLERLMISSDAFEVDVCDKC
GLMGYSGWCTTCKSAENIIKMTIPYAAKLLFQELLSMNIAPRLRLEDIFQ
Q

3D structure

• PDB: 7z1o Architecture of the yeast Pol III pre-termination complex and pausing mechanism on poly(dT) termination signals.
• Chain: B
• Resolution: 2.7 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	H456 Q708 K911 K919 H1029	H419 Q671 K863 K871 H981
BS02	dna	B	K212 T221 E226 R227 K228 N245 M312 R314 K393 R446 K448 M449 E450 R451 Q476 E478 K479	K175 T184 E189 R190 K191 N208 M275 R277 K356 R409 K411 M412 E413 R414 Q439 E441 K442
BS03	dna	B	T190 S438 T439 T723 G1053 R1054 R1061	T153 S401 T402 T686 G1005 R1006 R1013
BS04	ZN	B	C1095 C1098 C1107 C1110	C1047 C1050 C1059 C1062

Gene Ontology

Molecular Function

• GO:0001056 RNA polymerase III activity
• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0008270 zinc ion binding
• GO:0016779 nucleotidyltransferase activity
• GO:0032549 ribonucleoside binding
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0006383 transcription by RNA polymerase III
• GO:0006384 transcription initiation at RNA polymerase III promoter
• GO:0006386 termination of RNA polymerase III transcription
• GO:0042797 tRNA transcription by RNA polymerase III

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex
• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005666 RNA polymerase III complex

External links

• PDB: RCSB:7z1o, PDBe:7z1o, PDBj:7z1o
• PDBsum: 7z1o
• PubMed: 36070694
• UniProt: P22276|RPC2_YEAST DNA-directed RNA polymerase III subunit RPC2 (Gene Name=RET1)