Structure of PDB 7yei Chain B

Receptor sequence

>7yeiB (length=434) Species: 192952 (Methanosarcina mazei Go1)

LVPRGSHMNPKRIRALKSGKQGDGPVVYWMSRDQRAEDNWALLFSRAIAK
EANVPVVVVFCLTDEFLEAGIRQYEFMLKGLQELEVSLSRKKIPSFFLRG
DPGEKISRFVKDYNAGTLVTDFSPLRIKNQWIEKVISGISIPFFEVDAHN
VVPCWEASQKHEYAAHTFRPKLYALLPEFLEEFPELEPNSVTPDPLFEPW
HFEPGEKAAKKVMESFIADRLDSYGALRNDPTKNMLSNLSPYLHFGQISS
QRVVLEVEKAESNPGSKKAFLDEILIWKEISDNFCYYNPGYDGFESFPSW
AKESLNAHRNDVRSHIYTLEEFEAGKTHDPLWNASQMELLSTGKMHGYTR
MYWAKKILEWSESPEKALEIAICLNDRYELDGRDPNGYAGIAWSIGGVHD
RAWGEREVTGKIRYMSYEGCKRKFDVKLYIEKYS

3D structure

• PDB: 7yei Visualizing the DNA repair process by a photolyase at atomic resolution
• Chain: B
• Resolution: 2.7 Å

Enzymatic activity

• Enzyme Commision number: 4.1.99.3: deoxyribodipyrimidine photo-lyase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	H161 R256 E301 W305 Y376 M379 R429 W431 R441 Y442 M443 S444 G447 R450	H166 R228 E273 W277 Y348 M351 R401 W403 R413 Y414 M415 S416 G419 R422
BS02	dna	B	K155 Y158 W328 R429 A430 W431 E433 K439 R450	K160 Y163 W300 R401 A402 W403 E405 K411 R422
BS03	FAD	B	Y252 L264 S265 N266 S268 L271 E301 K372 R378 N403 D409 N414 G415	Y224 L236 S237 N238 S240 L243 E273 K344 R350 N375 D381 N386 G387

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003677 DNA binding
• GO:0003904 deoxyribodipyrimidine photo-lyase activity
• GO:0016829 lyase activity

Biological Process

• GO:0000719 photoreactive repair
• GO:0006281 DNA repair

External links

• PDB: RCSB:7yei, PDBe:7yei, PDBj:7yei
• PDBsum: 7yei
• PubMed: 38033054
• UniProt: Q8PYK9