Structure of PDB 7y7r Chain B

Receptor sequence

>7y7rB (length=897) Species: 5141 (Neurospora crassa)

AALRNIWPKFPKWHEAPLAVAWEVTRFMHKVDSLGLKYDPSTARDVTDIE
KPPNDVFVTAMTGNFESKGSAVVLSAVLDYNPDNSPLYLVKLKPLMFEQG
CRLTRRFGPDRFFEILIPSPTSSPSVPPVSKQAVEEVIQWLTMGQHSLVG
RQWRAFFAKDAGYRKPPIIKERVHFFAETGITFRPDEPVEQRTEFKVSQM
LDWLLQLDNNTWQPHLKLFSRIQLGLSKTYAIMTLEPHQIRHHKTDLLSP
SGTGEVMNDGVGRMSRSVAKRIRDVLGLGDVPSAVQGRFGSAKGMWVIDV
DDTGDEDWIETYPSQRKWECDFVDKHQRTLEVRSVASELKSAGLNLQLLP
VLEDRARDKVKMRQAIGDRLINDLQRQFSEQKHALNRPVEFRQWVYESYS
SRATRVSHGRVPFLAGLPDSQEETLNFLMNSGFDPKKQKYLQDIAWDLQK
RKCDTLKSKLNIRVGRSAYIYMIADFWGVLEENEVHVGFSSKFRDEESFT
LLSDCDVLVARSPAHFPSDIQRVRAVFKPELHSLKDVIIFSTKGDVPLAK
KLSGGDYDGDMAWVCWDPEIVDGFVNAEMPLEPDLSRYLKKDKTTFKQLM
ASHGTGSAAKEQTTYDMIQKSFHFALQPNFLGMCTNYKERLCYINNSVSN
KPAIILSSLVGNLVDQSKQGIVFNEASWAQLRRELLGGALSLPDPMYKSD
SWLGRGEPTHIIDYLKFSIARPAIDKELEAFHNMKAADGAHFWDPDLASY
YTFFKEISDKSRSSALLFTTLKNRIGEVEKEYGRLVKKEDPYPVRVNQVY
EKWCAITPSKVIRLLELSFLADREMNTWALLRASTAFKLYYHKSPKFVWQ
MAGRQLAYIKAQMTSRPGEGAPALMTAFMYAGLMPDKKFTKQYVARL

3D structure

• PDB: 7y7r Structural insights into the dual activities of the two-barrel RNA polymerase QDE-1.
• Chain: B
• Resolution: 2.1 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	K790 Y919 G1083 T1086 N1087 R1091 R1133 S1142	K340 Y469 G632 T635 N636 R640 R682 S691
BS02	rna	B	R591 S677 K678 Q736 R738 R783 D1011	R164 S227 K228 Q286 R288 R333 D560
BS03	CA	B	D1007 D1009 D1011	D556 D558 D560
BS04	CA	B	D1007 D1009	D556 D558
BS05	GTP	B	R671 K743 K767 D1009 K1119	R221 K293 K317 D558 K668

Gene Ontology

Molecular Function

• GO:0003968 RNA-dependent RNA polymerase activity

External links

• PDB: RCSB:7y7r, PDBe:7y7r, PDBj:7y7r
• PDBsum: 7y7r
• PubMed: 36039765
• UniProt: Q9Y7G6