Structure of PDB 7y7p Chain B

Receptor sequence

>7y7pB (length=908) Species: 5141 (Neurospora crassa)

VAARLRNIWPKFPKWLHEAPLAVAWEVTRLFMHCKVDLELKYDPSWSTAR
DVTDIWKTLYRLEKPPNDVFVTAMTGNFESKGSAVVLSAVLDYNPDNSPA
PLYLVKLKPLMFEQGCRLTRRFGPDRFFEILIPSPTSTSPSVPPVVSKQP
AVEEVIQWLTMGQHSLVGRQWRAFFAKDAGYRKPLRPIIKERVHFFAETG
ITFRPDEPVEQRTEFKVSQMLDWLLQLDNNTWQPHLKLFSRIQLGLSKTY
AIMTLEPHQIRHHKTDLLSPSGTGEVMNDGVGRMSRSVAKRIRDVLGLGD
VPSAVQGRFGSAKGMWVIDVDDTGDEDWIETYPSQRKWECDFVDKHQRTL
EVRSVASELKSAGLNLQLLPVLEDRARDKVKMRQAIGDRLINDLQRQFSE
QKHALNRPVEFRQWVYESYSSRATRVSHGRVPFLAGLPDSQEETLNFLMN
SGFDPKKQKYLQDIAWDLQKRKCDTLKSKLNIRVGRSAYIYMIADFWGVL
EENEVHVGFSSKFRDEEESFTLLSDCDVLVARSPAHFPSDIQRVRAVFKP
ELHSLKDVIIFSTKGDVPLAKKLSGGDYDGDMAWVCWDPEIVDGFVNAEM
PLEPDLSRYLKKDKTTFKQLMASHGTGSAAKEQTTYDMIQKSFHFALQPN
FLGMCTNYKERLCYINNSVSNKPAIILSSLVGNLVDQSKQGIVFNEASWA
QLRRELLGGALSLPDPMYKSDSWLGRGEPTHIIDYLKFSIARPAIDKELE
AFHNAEDGAHFWDPDLASYYTFFKEISDKSRSSALLFTTLKNRIGEVEKE
YGRPVRVNQVYEKWCAITPESKVIRLLELSFLADREMNTWALLRASTAFK
LYYHKSPKFVWQMAGRQLAYIKAQMTSRPGEGAPALMTAFMYAGLMPDKK
FTKQYVAR

3D structure

• PDB: 7y7p Structural insights into the dual activities of the two-barrel RNA polymerase QDE-1.
• Chain: B
• Resolution: 2.7 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	Y590 N795 Y919 S963 M1012 L1082 G1083 M1084 N1087 R1091	Y181 N365 Y489 S533 M582 L652 G653 M654 N657 R661
BS02	rna	B	R591 R611 S677 Q736 R738 R783 D1011	R182 R192 S247 Q306 R308 R353 D581
BS03	CA	B	D1007 D1009 D1011	D577 D579 D581
BS04	CA	B	D1007 D1009	D577 D579
BS05	ZAN	B	R671 K743 K767 R962 P964 D1007 D1009 V1115 D1116	R241 K313 K337 R532 P534 D577 D579 V685 D686

Gene Ontology

Molecular Function

• GO:0003968 RNA-dependent RNA polymerase activity

External links

• PDB: RCSB:7y7p, PDBe:7y7p, PDBj:7y7p
• PDBsum: 7y7p
• PubMed: 36039765
• UniProt: Q9Y7G6