Structure of PDB 7y6i Chain B

Receptor sequence

>7y6iB (length=469) Species: 9606 (Homo sapiens) [Search protein sequence]

GWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITG
LSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTV
GFAETVRDLLQEYGAPIVDPINDIRIIGVVSVTVLLAISLAGMEWESKAQ
VLFFLVIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVPDWRGP
DGTFFGMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISY
LAISATIGSCVVRDASGVLNDTVTPGWGACEGLACSYGWNFTECTQQHSC
HYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCED
QLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFF
LCSYALINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTW
WAALIAIGVVLFLLLYVIY

3D structure

PDB

7y6i Cryo-EM structure of the human sodium-chloride cotransporter NCC.

Chain

Resolution

2.85 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	CL	B	G152 P349 S350	G16 P213 S214
BS02	NA	B	N149 I150 H234 P349 T352	N13 I14 H98 P213 T216
BS03	CL	B	G353 L355 Y540	G217 L219 Y404
BS04	NA	B	L148 W151 G152 A464 S467 S468	L12 W15 G16 A328 S331 S332
BS05	Y01	B	T180 N566 W568 F572	T44 N430 W432 F436
BS06	Y01	B	L170 L177 P331 W333 F340 F341	L34 L41 P195 W197 F204 F205
BS07	Y01	B	W568 F572 I575 I576	W432 F436 I439 I440
BS08	LPE	B	T180 S183 Q563	T44 S47 Q427
BS09	LPE	B	V179 L376 M377 F380 T383 I384	V43 L240 M241 F244 T247 I248
BS10	LPE	B	L301 W381 I389	L165 W245 I253

Gene Ontology

	Molecular Function
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0008511	sodium:potassium:chloride symporter activity
GO:0015081	sodium ion transmembrane transporter activity
GO:0015293	symporter activity
GO:0015377	chloride:monoatomic cation symporter activity
GO:0015378	sodium:chloride symporter activity
GO:0022857	transmembrane transporter activity
GO:0046873	metal ion transmembrane transporter activity

	Biological Process
GO:0002021	response to dietary excess
GO:0006811	monoatomic ion transport
GO:0006814	sodium ion transport
GO:0006884	cell volume homeostasis
GO:0007165	signal transduction
GO:0035725	sodium ion transmembrane transport
GO:0055064	chloride ion homeostasis
GO:0055075	potassium ion homeostasis
GO:0055078	sodium ion homeostasis
GO:0055085	transmembrane transport
GO:0070294	renal sodium ion absorption
GO:1902476	chloride transmembrane transport
GO:1904044	response to aldosterone
GO:1990573	potassium ion import across plasma membrane

	Cellular Component
GO:0005829	cytosol
GO:0005886	plasma membrane
GO:0016020	membrane
GO:0016324	apical plasma membrane
GO:0070062	extracellular exosome

View graph for
Molecular Function

View graph for
Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:7y6i, PDBe:7y6i, PDBj:7y6i
PDBsum	7y6i
PubMed	36351028
UniProt	P55017\|S12A3_HUMAN Solute carrier family 12 member 3 (Gene Name=SLC12A3)

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