Structure of PDB 7y0p Chain B

Receptor sequence

>7y0pB (length=451) Species: 1348623 (Priestia megaterium NBRC 15308 = ATCC 14581)

KEMPQPKTFGELKNLPLLNTDKPVQALMKIADELGEIFKFEAPGRVTRYL
SSQRLIKEACDESRFDKNLSQALKFVRDFTGDGLATSWTHEKNWKKAHNI
LLPSFSQQAMKGYHAMMVDIAVQLVQKWERLNADEHIEVPEDMTRLTLDT
IGLCGFNYRFNSFYRDQPHPFITSMVRALDEAMNKLQRPDDPAYDENKRQ
FQEDIKVMNDLVDKIIADRKASGEQSDDLLTHMLNGKDPETGEPLDDENI
RYQIITFLLAGHEVTSGLLSFALYFLVKNPHVLQKAAEEAARVLVDPVPS
YKQVKQLKYVGMVLNEALRLWPTAPAFSLYAKEDTVLGGEYPLEKGDELM
VLIPQLHRDKTIWGDDVEEFRPERFENPSAIPQHAFKPFGNGQRACIGQQ
FALHEATLVLGMMLKHFDFEDHTNYELDIKETLTLKPEGFVVKAKSKKIP
L

3D structure

• PDB: 7y0p Crystal structure of the P450 BM3 heme domain mutant F87A/T268V/A82T/I263L in complex with N-imidazolyl-hexanoyl-L-phenylalanine, p-cresol and hydroxylamine
• Chain: B
• Resolution: 1.99 Å

Enzymatic activity

• Enzyme Commision number: 1.14.14.1: unspecific monooxygenase.
  1.6.2.4: NADPH--hemoprotein reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEM	B	K69 L86 A87 W96 F107 I153 F261 A264 V268 T269 L272 A328 F331 P392 F393 R398 C400 I401 G402 A406	K67 L84 A85 W94 F105 I151 F257 A260 V264 T265 L268 A324 F327 P388 F389 R394 C396 I397 G398 A402
BS02	HOA	B	A264 V268	A260 V264
BS03	IC6	B	P25 Y51 L188 A330 M354 L437	P23 Y49 L186 A326 M350 L433
BS04	IC6	B	L20 R47 Y51 S72 Q73 A74 A328 A330	L18 R45 Y49 S70 Q71 A72 A324 A326
BS05	PCR	B	Y160 H171 F173 L215 I219	Y158 H169 F171 L211 I215

Gene Ontology

Molecular Function

• GO:0004497 monooxygenase activity
• GO:0005506 iron ion binding
• GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
• GO:0020037 heme binding

External links

• PDB: RCSB:7y0p, PDBe:7y0p, PDBj:7y0p
• PDBsum: 7y0p
• UniProt: P14779|CPXB_PRIM2 Bifunctional cytochrome P450/NADPH--P450 reductase (Gene Name=cyp102A1)