Structure of PDB 7xk6 Chain B

Receptor sequence

>7xk6B (length=412) Species: 345073 (Vibrio cholerae O395)

GLKKFLEDIEHHFEPGGKHEKWFALYEAAATLFYTPGLVTKRSSHVRDSV
DLKRIMIMVWLAVFPAMFWGMYNAGGQAIAALNHLYSGDQLAAIVAGNWH
YWLTEMLGGTMSSDAGWGSKMLLGATYFLPIYATVFIVGGFWEVLFCMVR
KHEVNEGFFVTSILFALIVPPTLPLWQAALGITFGVVVAKEVFGGTGRNF
LNPALAGRAFLFFAYPAQISGDLVWTAADGYSGATALSQWAQGGAGALIN
NATGQTITWMDAFIGNIPGSIGEVSTLALMIGAAFIVYMGIASWRIIGGV
MIGMILLSTLFNVIGSDTNAMFNMPWHWHLVLGGFAFGMFFMATDPVSAS
FTNSGKWAYGILIGVMCVLIRVVNPAYPEGMMLAILFANLFAPLFDHVVV
ERNIKRRLARYG

3D structure

• PDB: 7xk6 Cryo-EM structures of Na + -pumping NADH-ubiquinone oxidoreductase from Vibrio cholerae.
• Chain: B
• Resolution: 3.0 Å

Enzymatic activity

• Enzyme Commision number: 7.2.1.1: NADH:ubiquinone reductase (Na(+)-transporting).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FMN	B	R209 F213 W226 T236 S271 E274 E380 G381 M383	R208 F212 W225 T235 S270 E273 E379 G380 M382
BS02	RBF	B	V60 T162 L165 K191 N200 N203 P204 F342 M343 D346 P347	V59 T161 L164 K190 N199 N202 P203 F341 M342 D345 P346
BS03	0NI	B	K54 E157 F159 F160	K53 E156 F158 F159
BS04	FMN	B	F213 P217 A377	F212 P216 A376

Gene Ontology

Molecular Function

• GO:0010181 FMN binding
• GO:0016655 oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
• GO:1902444 riboflavin binding

Biological Process

• GO:0006814 sodium ion transport
• GO:0022904 respiratory electron transport chain
• GO:0055085 transmembrane transport

Cellular Component

• GO:0005886 plasma membrane
• GO:0016020 membrane

External links

• PDB: RCSB:7xk6, PDBe:7xk6, PDBj:7xk6
• PDBsum: 7xk6
• PubMed: 35882843
• UniProt: A5F5X0|NQRB_VIBC3 Na(+)-translocating NADH-quinone reductase subunit B (Gene Name=nqrB)