Structure of PDB 7thn Chain B

Receptor sequence

>7thnB (length=478) Species: 104623 (Prodigiosinella confusarubida)

TISTPVIIDSLIRHAQRTPEQTALLCGDQHWNYRQLVTRAHVMASALRQA
GLSGQAILLNLPKSLDAVAAIYATWLSGNHYIPIDYSQPSSRIERIIAAA
APALIIDTAWLATLDSQVYHNPIAAILYTSGSTGTPKGVQISHEMLGFFI
QWAVRDTQLTARDVLSNHASFAFDLSTFDLFASAYVGAATWIIRESEQKD
CAALAQGLQRHAVSVWYSVPSILAMLEKSTLLNPTLGQSLRQVIFAGEPY
PVTALKRLLPCLPQPCRVSNWYGPTETNVCVAYAIDRARLAMLKQVPIGL
PLEGLTAQLEDENGDRHPLTAQLRLSGELLISGPCVTPGYSNVVVPRQAA
LHPHQCHATGDWVEMTPEGLVFRGRIDDMVKINGYRVELGEIESVLHQHP
AIDRAALCVELGDLRQTLIMVISLQTGAVPPGLLELKQFLQQKLPSYMIP
NKLVITESLPVNAKVDRKQLAGVVAVLE

3D structure

• PDB: 7thn Essential Role of Loop Dynamics in Type II NRPS Biomolecular Recognition.
• Chain: B
• Resolution: 1.6 Å

Enzymatic activity

• Enzyme Commision number: 6.2.1.53: L-proline--[L-prolyl-carrier protein] ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	AZI	B	P277 Q278	P263 Q264
BS02	AZI	B	W165 R168	W152 R155
BS03	AZI	B	T267 R271	T253 R257
BS04	AZI	B	V468 I469	V454 I455
BS05	AZI	B	R93 Y141 T142	R92 Y128 T129
BS06	MG	B	F186 D187 T289	F173 D174 T275
BS07	I5M	B	D187 L188 V233 I236 A260 G261 E262 P263 N284 W285 Y286 G287 P288 T289 D375 R389 K395 N397 G398 Y399 R400	D174 L175 V219 I222 A246 G247 E248 P249 N270 W271 Y272 G273 P274 T275 D361 R375 K381 N383 G384 Y385 R386

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0016874 ligase activity
• GO:0031177 phosphopantetheine binding

Biological Process

• GO:0017000 antibiotic biosynthetic process
• GO:0043041 amino acid activation for nonribosomal peptide biosynthetic process
• GO:0044550 secondary metabolite biosynthetic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:7thn, PDBe:7thn, PDBj:7thn
• PDBsum: 7thn
• PubMed: 36173802
• UniProt: Q5W263|PIGI_SERS3 L-proline--[L-prolyl-carrier protein] ligase (Gene Name=pigI)