Structure of PDB 7th4 Chain B

Receptor sequence
>7th4B (length=291) Species: 300852 (Thermus thermophilus HB8) [Search protein sequence]
MKIRDLLKARRGPLFSFEFFPPKDPEGEEALFRTLEELKAFRPAFVSITY
GAMGSTRERSVAWAQRIQSLGLNPLAHLTVAGQSRKEVAEVLHRFVESGV
ENLLALRGDPPRGERVFRPHPEGFRYAAELVALIRERYGDRVSVGGAAYP
EGHPESESLEADLRHFKAKVEAGLDFAITQLFFNNAHYFGFLERARRAGI
GIPILPGIMPVTSYRQLRRFTEVCGASIPGPLLAKLERHQDDPKAVLEIG
VEHAVRQVAELLEAGVEGVHFYTLNKSPATRMVLERLGLRP
3D structure
PDB7th4 5-Formyltetrahydrofolate promotes conformational remodeling in a methylenetetrahydrofolate reductase active site and inhibits its activity.
ChainB
Resolution1.45 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 1.5.1.54: methylenetetrahydrofolate reductase (NADH).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FAD B T49 Y50 H77 T79 L106 R107 G108 D109 Y126 A127 A147 Y149 H153 S156 H165 I178 Q180 Y272 T49 Y50 H77 T79 L106 R107 G108 D109 Y126 A127 A147 Y149 H153 S156 H165 I178 Q180 Y272
BS02 FFO B F20 T49 D109 Q180 M209 Q216 R219 Y272 F20 T49 D109 Q180 M209 Q216 R219 Y272
Gene Ontology
Molecular Function
GO:0000166 nucleotide binding
GO:0004489 methylenetetrahydrofolate reductase (NAD(P)H) activity
GO:0016491 oxidoreductase activity
GO:0071949 FAD binding
GO:0106312 methylenetetrahydrofolate reductase (NADH) activity
Biological Process
GO:0006555 methionine metabolic process
GO:0009086 methionine biosynthetic process
GO:0035999 tetrahydrofolate interconversion
Cellular Component
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:7th4, PDBe:7th4, PDBj:7th4
PDBsum7th4
PubMed36592927
UniProtQ5SLG6

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