Structure of PDB 7t7k Chain B

Receptor sequence

>7t7kB (length=438) Species: 4896 (Schizosaccharomyces pombe)

MKFLNPPFPYSMTSDPESFGHECFTRRWGIILTGIEKDVSERLSKLASTS
KDSEVVAQGKPLLNDLEAFKSDIKNDRPLVPLEGEGQDIVEYNEELKQLD
NASWGNAPWLYSECYYYRRISLIFARYSEWKAYDPFFQQKDSTLKSSRAA
VEELAGRYCLLEEELNSIAKKGDSHIAYMVFVEMAQISLWGNATDLSLLT
NLSYEELQNLQGQKVVEESQKNILVNDFPTVWSKLKDVHNGRIDFVLDNA
GFELYVDLIFAAYLLKAGIAKEIVLHPKDFPWFVSDVLPYDIEYLLTNLD
TIFPTESVTKFATDLRSFSAKGQLRLRTDPFWTTAHYFGRMPDFAAGLLT
ELEKSDMIFFKGDLNYRKLTGDCLWPRTTPFGKTLGPIANAINACALRTC
KADVVVGLPDGLYEKIAKDLPHWERTGKYAVVEFCPKA

3D structure

• PDB: 7t7k Fission yeast Duf89 and Duf8901 are cobalt/nickel-dependent phosphatase-pyrophosphatases that act via a covalent aspartyl-phosphate intermediate.
• Chain: B
• Resolution: 1.9 Å

Enzymatic activity

• Enzyme Commision number: 3.1.3.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PO4	B	S71 K74	S71 K74
BS02	PO4	B	S44 S48	S44 S48
BS03	CO	B	D195 N249 F283 D286	D195 N249 F283 D286
BS04	CO	B	G172 S174	G172 S174

Gene Ontology

Molecular Function

• GO:0004427 inorganic diphosphate phosphatase activity
• GO:0016787 hydrolase activity
• GO:0016791 phosphatase activity
• GO:0046872 metal ion binding
• GO:0097023 fructose 6-phosphate aldolase activity
• GO:0103026 fructose-1-phosphatase activity

Biological Process

• GO:0006974 DNA damage response
• GO:0030643 intracellular phosphate ion homeostasis
• GO:1990748 cellular detoxification

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:7t7k, PDBe:7t7k, PDBj:7t7k
• PDBsum: 7t7k
• PubMed: 35314193
• UniProt: Q9UT55|ART1A_SCHPO Damage-control phosphatase SPAC806.04c (Gene Name=SPAC806.04c)