Structure of PDB 7t0e Chain B

Receptor sequence

>7t0eB (length=485) Species: 235443 (Cryptococcus neoformans var. grubii H99)

ATEFTPSVYSLVSKPLPSNSRPSATLDEQAETEDLISQLFDLTADPNALV
SEHGKRYSGLRKQEHTQFLASSFFQLPGKFVSLDASRPWLVFWTVHSLDL
LGVALDQGTKDRVVSTLLHFLSPKGGFGGGPANSQIPHLLPTYASVCSLA
IAGNDSSTGGWKDLAAARQSIYEFFMRCKRPDGGFVVCEGGEVDVRGTYC
LLVVATLLDIITPELLHNVDKFVSACQTYEGGFACASFPFPEPSCRVSMA
EAHGGYTSCSLNSHFLLTSVPLPSFPLSIDANAALRWTVLQQGEPIEGGG
FRGRTNKLVDGCYSWWVGGGAPVAEELVRREKSRKVIPPIFNRVALQEFT
LVAAQQDPGSTGGLRDKPGKRPDQYHTCNNLSGLSIAQHKMSHSPSTVSS
NRLKFDASKGLPAVKPVAPGGGWKNEDERQNARREIWANALGWIEEEGGE
IIVGGKDNRINTTTPVFNILGLRLKPFINYFYCQE

3D structure

• PDB: 7t0e Structure-Guided Discovery of Potent Antifungals that Prevent Ras Signaling by Inhibiting Protein Farnesyltransferase.
• Chain: B
• Resolution: 2.223 Å

Enzymatic activity

• Enzyme Commision number: 2.5.1.58: protein farnesyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	B	D323 C325 H410	D310 C312 H376
BS02	3FX	B	G489 K490 D491	G455 K456 D457
BS03	3FX	B	R62 Q64 E65	R61 Q63 E64
BS04	3FX	B	S123 P124 K125 A133 N134 S135	S122 P123 K124 A132 N133 S134
BS05	FPP	B	H266 G268 Y269 C272 R317 K320 Y326 W329	H253 G255 Y256 C259 R304 K307 Y313 W316
BS06	XOA	B	S87 W90 C325 Y409 H410	S86 W89 C312 Y375 H376

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004659 prenyltransferase activity
• GO:0004660 protein farnesyltransferase activity
• GO:0008270 zinc ion binding
• GO:0008318 protein prenyltransferase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0018343 protein farnesylation
• GO:0097354 prenylation

Cellular Component

• GO:0005965 protein farnesyltransferase complex

External links

• PDB: RCSB:7t0e, PDBe:7t0e, PDBj:7t0e
• PDBsum: 7t0e
• PubMed: 36218371
• UniProt: T2BPA1