Structure of PDB 7t0c Chain B

Receptor sequence

>7t0cB (length=487) Species: 235443 (Cryptococcus neoformans var. grubii H99)

ATEFTPSVYSLVSKPLPSNSRPSATLDEQAETEDLISQLFDLTADPNALV
SEHGKRYSGLRKQEHTQFLASSFFQLPGKFVSLDASRPWLVFWTVHSLDL
LGVALDQGTKDRVVSTLLHFLSPKGGFGGGPANSQIPHLLPTYASVCSLA
IAGNDSSTGGWKDLAAARQSIYEFFMRCKRPDGGFVVCEGGEVDVRGTYC
LLVVATLLDIITPELLHNVDKFVSACQTYEGGFACASFPFPSSEPSCRVS
MAEAHGGYTSCSLNSHFLLTSVPLPSFPLSIDANAALRWTVLQQGEPIEG
GGFRGRTNKLVDGCYSWWVGGGAPVAEELVRREKSRKVIPPIFNRVALQE
FTLVAAQQDPGSTGGLRDKPGKRPDQYHTCNNLSGLSIAQHKMSHSPSTV
SSNRLKFDASKGLPAVKPVAPGGGWKNEDERQNARREIWANALGWIEEEG
GEIIVGGKDNRINTTTPVFNILGLRLKPFINYFYCQE

3D structure

• PDB: 7t0c Structure-Guided Discovery of Potent Antifungals that Prevent Ras Signaling by Inhibiting Protein Farnesyltransferase.
• Chain: B
• Resolution: 1.903 Å

Enzymatic activity

• Enzyme Commision number: 2.5.1.58: protein farnesyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	B	D323 C325 H410	D312 C314 H378
BS02	XN4	B	W90 W94 D323 C325 D407 Y409 H410	W89 W93 D312 C314 D375 Y377 H378
BS03	3FX	B	G489 K490 D491	G457 K458 D459
BS04	3FX	B	R62 Q64 E65	R61 Q63 E64
BS05	3FX	B	K125 A133 N134 S135	K124 A132 N133 S134
BS06	FPP	B	R197 H266 G268 Y269 C272 R317 K320 Y326 W329	R196 H255 G257 Y258 C261 R306 K309 Y315 W318

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004659 prenyltransferase activity
• GO:0004660 protein farnesyltransferase activity
• GO:0008270 zinc ion binding
• GO:0008318 protein prenyltransferase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0018343 protein farnesylation
• GO:0097354 prenylation

Cellular Component

• GO:0005965 protein farnesyltransferase complex

External links

• PDB: RCSB:7t0c, PDBe:7t0c, PDBj:7t0c
• PDBsum: 7t0c
• PubMed: 36218371
• UniProt: T2BPA1