Structure of PDB 7t0a Chain B

Receptor sequence

>7t0aB (length=486) Species: 235443 (Cryptococcus neoformans var. grubii H99)

ATEFTPSVYSLVSKPLPSNSRPSATLDEQAETEDLISQLFDLTADPNALV
SEHGKRYSGLRKQEHTQFLASSFFQLPGKFVSLDASRPWLVFWTVHSLDL
LGVALDQGTKDRVVSTLLHFLSPKGGFGGGPANSQIPHLLPTYASVCSLA
IAGNDSSTGGWKDLAAARQSIYEFFMRCKRPDGGFVVCEGGEVDVRGTYC
LLVVATLLDIITPELLHNVDKFVSACQTYEGGFACASFPFPSEPSCRVSM
AEAHGGYTSCSLNSHFLLTSVPLPSFPLSIDANAALRWTVLQQGEPIEGG
GFRGRTNKLVDGCYSWWVGGGAPVAEELVRREKSRKVIPPIFNRVALQEF
TLVAAQQDPGSTGGLRDKPGKRPDQYHTCNNLSGLSIAQHKMSHSPSTVS
SNRLKFDASKGLPAVKPVAPGGGWKNEDERQNARREIWANALGWIEEEGG
EIIVGGKDNRINTTTPVFNILGLRLKPFINYFYCQE

3D structure

• PDB: 7t0a Structure-Guided Discovery of Potent Antifungals that Prevent Ras Signaling by Inhibiting Protein Farnesyltransferase.
• Chain: B
• Resolution: 2.099 Å

Enzymatic activity

• Enzyme Commision number: 2.5.1.58: protein farnesyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	B	D323 C325 H410	D311 C313 H377
BS02	3FX	B	Y58 G489 K490 D491	Y57 G456 K457 D458
BS03	3FX	B	R62 Q64 E65	R61 Q63 E64
BS04	3FX	B	S123 P124 K125 A133 N134 S135 Q136	S122 P123 K124 A132 N133 S134 Q135
BS05	XMY	B	W90 R197 H266 G268 C325 Y326 W329 Y409	W89 R196 H254 G256 C313 Y314 W317 Y376

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004659 prenyltransferase activity
• GO:0004660 protein farnesyltransferase activity
• GO:0008270 zinc ion binding
• GO:0008318 protein prenyltransferase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0018343 protein farnesylation
• GO:0097354 prenylation

Cellular Component

• GO:0005965 protein farnesyltransferase complex

External links

• PDB: RCSB:7t0a, PDBe:7t0a, PDBj:7t0a
• PDBsum: 7t0a
• PubMed: 36218371
• UniProt: T2BPA1