Structure of PDB 7t09 Chain B

Receptor sequence

>7t09B (length=497) Species: 235443 (Cryptococcus neoformans var. grubii H99)

ATEFTPSVYSLVSKPLPSNSRPSATLDEQAETEDLISQLFDLTADPNALV
SEHGKRYSGLRKQEHTQFLASSFFQLPGKFVSLDASRPWLVFWTVHSLDL
LGVALDQGTKDRVVSTLLHFLSPKGGFGGGPANSQIPHLLPTYASVCSLA
IAGNDSSTGGWKDLAAARQSIYEFFMRCKRPDGGFVVCEGGEVDVRGTYC
LLVVATLLDIITPELLHNVDKFVSACQTYEGGFACASFPFPSVVPSTSAF
PTSEPSCRVSMAEAHGGYTSCSLNSHFLLTSVPLPSFPLSIDANAALRWT
VLQQGEPIEGGGFRGRTNKLVDGCYSWWVGGGAPVAEELVRREKSRKVIP
PIFNRVALQEFTLVAAQQDPGSTGGLRDKPGKRPDQYHTCNNLSGLSIAQ
HKMSHSPSTVSSNRLKFDASKGLPAVKPVAPGGGWKNEDERQNARREIWA
NALGWIEEEGGEIIVGGKDNRINTTTPVFNILGLRLKPFINYFYCQE

3D structure

• PDB: 7t09 Structure-Guided Discovery of Potent Antifungals that Prevent Ras Signaling by Inhibiting Protein Farnesyltransferase.
• Chain: B
• Resolution: 1.984 Å

Enzymatic activity

• Enzyme Commision number: 2.5.1.58: protein farnesyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	B	D323 C325 H410	D322 C324 H388
BS02	3FX	B	G489 K490 D491	G467 K468 D469
BS03	3FX	B	R62 Q64 E65	R61 Q63 E64
BS04	3FX	B	K125 A133 S135 Q136	K124 A132 S134 Q135
BS05	XMV	B	W90 R197 D323 C325 D407 Y409 H410	W89 R196 D322 C324 D385 Y387 H388
BS06	FPP	B	R197 Y200 H266 G268 Y269 C272 R317 K320 Y326 W329	R196 Y199 H265 G267 Y268 C271 R316 K319 Y325 W328

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004659 prenyltransferase activity
• GO:0004660 protein farnesyltransferase activity
• GO:0008270 zinc ion binding
• GO:0008318 protein prenyltransferase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0018343 protein farnesylation
• GO:0097354 prenylation

Cellular Component

• GO:0005965 protein farnesyltransferase complex

External links

• PDB: RCSB:7t09, PDBe:7t09, PDBj:7t09
• PDBsum: 7t09
• PubMed: 36218371
• UniProt: T2BPA1