Structure of PDB 7sfl Chain B

Receptor sequence

>7sflB (length=830) Species: 9606 (Homo sapiens)

WIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVLVIMMATVVTTITGL
STSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVG
FAETVVELLKEHSILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQI
VLLVILLLAIGDFVIGTFIPLESKKPKGFFGYKSEIFNENFGPDFREEET
FFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTLVYVGI
AVSVGSCVVRDATGNVNDTIVTELTNCTSAACKLNFDFSSCESSPCSYGL
MNNFQVMSMVSGFTPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYP
AFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASY
ALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAAL
LTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKN
FRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMS
IDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVL
GFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLKEGLKNTIDVWWLF
DDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFR
IDFSDIMVLGDINTKPKKENIIAFEEIIEPYRLHEDDKEQDIADKMKEDE
PWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSS
ALYMAWLEALSKDLPPILLVRGNHQSVLTF

3D structure

• PDB: 7sfl Inhibition mechanism of NKCC1 involves the carboxyl terminus and long-range conformational coupling.
• Chain: B
• Resolution: 3.87 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FUN	B	M794 G796 R801 H822 V823 M825 L863 G887 Y903	M508 G510 R515 H536 V537 M539 L577 G601 Y617

Gene Ontology

Molecular Function

• GO:0015377 chloride:monoatomic cation symporter activity
• GO:0022857 transmembrane transporter activity

Biological Process

• GO:0006811 monoatomic ion transport
• GO:0055085 transmembrane transport

Cellular Component

• GO:0016020 membrane

External links

• PDB: RCSB:7sfl, PDBe:7sfl, PDBj:7sfl
• PDBsum: 7sfl
• PubMed: 36306358
• UniProt: P55011|S12A2_HUMAN Solute carrier family 12 member 2 (Gene Name=SLC12A2)