Structure of PDB 7rcm Chain B

Receptor sequence

>7rcmB (length=391) Species: 9606 (Homo sapiens)

AALRQPQVAELLAEARRAFREEFGAEPELAVSAPGRVNLIGEHTDYNQGL
VLPMALELMTVLVGSPRKDGLVSLLTTSEGADEPQRLQFPLPTAQRSLEP
GTPRWANYVKGVIQYYPAAPLPGFSAVVVSSVPLGGGLSSSASLEVATYT
FLQQLCPDSGTIAARAQVCQQAEHSFAGMPCGIMDQFISLMGQKGHALLI
DCRSLETSLVPLSDPKLAVLITNSNVRHSLASSEYPVRRRQCEEVARALG
AASLREVQLEELEAARDLVSKEGFRRARHVVGEIRRTAQAAAALRRGDYR
AFGRLMVESHRSLRDDYEVSCPELDQLVEAALAVPGVYGSRMTGGGFGGC
TVTLLEASAAPHAMRHIQEHYGGTATFYLSQAADGAKVLCL

3D structure

• PDB: 7rcm Structure-Based Optimization of Small Molecule Human Galactokinase Inhibitors.
• Chain: B
• Resolution: 2.1 Å

Enzymatic activity

• Enzyme Commision number: 2.7.1.6: galactokinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADP	B	T77 S79 W106 Y109 L135 G136 G138 S140 S141 S142 L145	T76 S78 W105 Y108 L134 G135 G137 S139 S140 S141 L144
BS02	GLA	B	E43 H44 D46 G183 D186 Y236 G346	E42 H43 D45 G182 D185 Y235 G345
BS03	PO4	B	H363 H367	H362 H366

Gene Ontology

Molecular Function

• GO:0004335 galactokinase activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0005534 galactose binding
• GO:0016301 kinase activity
• GO:0016773 phosphotransferase activity, alcohol group as acceptor

Biological Process

• GO:0006012 galactose metabolic process
• GO:0016310 phosphorylation
• GO:0019402 galactitol metabolic process
• GO:0033499 galactose catabolic process via UDP-galactose
• GO:0046835 carbohydrate phosphorylation
• GO:0061623 glycolytic process from galactose

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0016020 membrane
• GO:0070062 extracellular exosome

External links

• PDB: RCSB:7rcm, PDBe:7rcm, PDBj:7rcm
• PDBsum: 7rcm
• PubMed: 34491744
• UniProt: P51570|GALK1_HUMAN Galactokinase (Gene Name=GALK1)