Structure of PDB 7r1f Chain B

Receptor sequence

>7r1fB (length=728) Species: 1601067 (Influenza B virus (B/Memphis/13/2003))

GMNINPYFLFIDVPIQAAISTTFPYTGVPPYSHGTGTGYTIDTVIRTHEY
SNKGKQYISDVTGCTMVDPTNGPLPEDNEPSAYAQLDCVLEALDRMDEEH
PGLFQAASQNAMETLMVTTVDKLTQGRQTFDWTVCRNQPAATALNTTITS
FRLNDLNGADKGGLIPFCQDIIDSLDRPEMTFFSVKNIKKKLPAFLIKRI
PMKVKDKITKVEYIKRALSLNTMTKDAERGKLKRRAIATAGIQIRGFVLV
VENLAKNICENLEQSGLPVGGNEKKAKLSNAVAKMLSNCPPGGISMTVTG
DNTKWNECLNPRIFLAMTERITRDSPIWFRDFCSIAPVLFSNKIARLGKG
FMITSKTKRLKAQIPCPDLFSIPLERYNEETRAKLKKLKPFFNEEGTASL
SPGMMMGMFNMLSTVLGVAALGIKNIGNKEYLWDGLQSSDDFALFVNAKD
EETCMEGINDFYRTCKLLGINMSKKKSYCNETGMFEFTSMFYRDGFVSNF
AMELPSFGVAGVNESADMAIGMTIIKNNMINNGMGPATAQTAIQLFIADY
RYTYKCHRGDSKVEGKRMKIIKELWENTKGRDGLLVADGGPNIYNLRNLH
IPEIVLKYNLMDPEYKGRLLHPQNPFVGHLSIMDYDAVSGTHSWRTKRNR
SILNTDQRNMILEEQCYAKCCNLFEACFNSASYRKPVGQHSMLEAMAHRL
RMDARLDYESGRMSKDDFEKAMAHLGEI

3D structure

• PDB: 7r1f Direct observation of backtracking by influenza A and B polymerases upon consecutive incorporation of the nucleoside analog T1106.
• Chain: B
• Resolution: 2.58 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	H32 G33 T34 L200 K365 E384 L674 N675	H33 G34 T35 L196 K361 E380 L653 N654
BS02	rna	B	R126 Q127 N136 K229 D230 I241 A242 R249 E256 P272 G274 G275 K353 M410 G411 M415 I524 N531	R127 Q128 N137 K225 D226 I237 A238 R245 E252 P268 G270 G271 K349 M406 G407 M411 I520 N527
BS03	rna	B	Y24 Y38 T42 R45 Q124 R126 K237 R238 R239 A240 K308 N310 G411 D444 S493 M506 P509	Y25 Y39 T43 R46 Q125 R127 K233 R234 R235 A236 K304 N306 G407 D440 S489 M502 P505
BS04	MG	B	D305 N306 D444	D301 N302 D440
BS05	MG	B	D305 D444	D301 D440
BS06	MG	B	G304 D445	G300 D441

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019083 viral transcription
• GO:0039523 symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity
• GO:0039694 viral RNA genome replication

Cellular Component

• GO:0030430 host cell cytoplasm
• GO:0042025 host cell nucleus

External links

• PDB: RCSB:7r1f, PDBe:7r1f, PDBj:7r1f
• PDBsum: 7r1f
• PubMed: 36596301
• UniProt: Q5V8Y6