Structure of PDB 7r0f Chain B

Receptor sequence

>7r0fB (length=401) Species: 83333 (Escherichia coli K-12)

MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEKNVLIDT
VKHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPI
YCTANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPD
SMMTYLTGDAVLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILT
PFSRLVTPKITEILGFNLPVDMIATSHGVVWRDNPTQIVELYLKWAADYQ
EDRITIFYDTMSNNTRMMADAIAQGIAETDPRVAVKIFNVARSDKNEILT
NVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLRFRNKRASAFGSHGWSG
GAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALKLCREHGREIARQWALAP
L

3D structure

• PDB: 7r0f Crystal structure of a flavodiiron protein D52K mutant in the oxidized state from Escherichia coli
• Chain: B
• Resolution: 1.978 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FMN	B	T260 M261 S262 N264 T265 S313 T314 M315 N316 N317 S345 H346 G347 W348 G350 W375	T260 M261 S262 N264 T265 S313 T314 M315 N316 N317 S345 H346 G347 W348 G350 W375
BS02	FE	B	D83 H84 D166 H227	D83 H84 D166 H227
BS03	FE	B	H79 E81 H147 D166	H79 E81 H147 D166
BS04	O	B	H79 E81 D83 H84 D166	H79 E81 D83 H84 D166
BS05	OXY	B	H23 H147	H23 H147

Gene Ontology

Molecular Function

• GO:0005506 iron ion binding
• GO:0009055 electron transfer activity
• GO:0010181 FMN binding
• GO:0016491 oxidoreductase activity
• GO:0016661 oxidoreductase activity, acting on other nitrogenous compounds as donors
• GO:0016966 nitric oxide reductase activity
• GO:0042802 identical protein binding
• GO:0046872 metal ion binding

Biological Process

• GO:0046210 nitric oxide catabolic process
• GO:0071731 response to nitric oxide

Cellular Component

• GO:0005737 cytoplasm
• GO:0032991 protein-containing complex

External links

• PDB: RCSB:7r0f, PDBe:7r0f, PDBj:7r0f
• PDBsum: 7r0f
• UniProt: Q46877|NORV_ECOLI Anaerobic nitric oxide reductase flavorubredoxin (Gene Name=norV)