Structure of PDB 7qtl Chain B

Receptor sequence

>7qtlB (length=717) Species: 1318616 (Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))

MDVNPTLLFLKVPVQNAISTTFPYTGDPPYSHGTGTGYTMDTVNRTHKYS
EKGKWTTNTETGAPQLNPIDGPLPEDNEPSGYAQTDCVLEAMAFLEESHP
GIFENSCLETMEIVQQTRVDKLTQGRQTYDWTLNRNQPAATALANTIEVF
RSNGLTANESGRLIDFLKDVMDSMDKEEMEITTHKKKQRLNKRSYLIRAL
TLNTMTKDAERGKLKRRAIATPGMQIRGFVYFVEALARSICEKLEQSGLP
VGGNEKKAKLANVVRKMMTNSQDTELSFTITGDNTKWNENQNPRMFLAMI
TYITRNQPEWFRNVLSIAPIMFSNKMARLGKGYMFESKSMKLRTQVPAEM
LANIDLKYFNKSTREKIEKIRPLLIDGTASLSPGMMMGMFNMLSTVLGVS
ILNLGQKKYTKTTYWWDGLQSSDDFALIVNAPNHEGIQAGVDRFYRTCKL
VGINMSKKKSYINRTGTFEFTSFFYRYGFVANFSMELPSFGVSGINESAD
MSVGVTVIKNNMINNDLGPATAQMALQLFIKDYRYTYRCHRGDTQIQTRR
AFELKKLWEQTRSKAGLLVSDGGPNLYNIRNLHIPEVCLKWELMDEDYQG
RLCNPMNPFVSHKEISMEYDAVATTHSWIPKRNRSNTSQRGILEDEQMYQ
KCCNLFEKFFPSSSYRRPVGISSMVEAMVSRARIDARIDFESGRIKKEEF
AEIMKICSTIEELRRQK

3D structure

• PDB: 7qtl Direct observation of backtracking by influenza A and B polymerases upon consecutive incorporation of the nucleoside analog T1106.
• Chain: B
• Resolution: 2.48 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	H32 G33 T34 M356 R365	H32 G33 T34 M334 R343
BS02	rna	B	Y30 G125 R126 Q127 R135 N136 M227 K229 I241 A242 T243 R249 R260 P272 G274 K353 Q367 P369 M409 G410 M411 N413 T528 N532	Y30 G125 R126 Q127 R135 N136 M205 K207 I219 A220 T221 R227 R238 P250 G252 K331 Q345 P347 M387 G388 M389 N391 T506 N510
BS03	rna	B	Y24 E264 N413 D445 D446 S494 M507 P510 R706	Y24 E242 N391 D423 D424 S472 M485 P488 R666
BS04	2TM	B	K235 R239 K308 N310 M409 G410 D445 K481	K213 R217 K286 N288 M387 G388 D423 K459
BS05	MG	B	G304 D305	G282 D283

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019083 viral transcription
• GO:0039523 symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity
• GO:0039694 viral RNA genome replication

Cellular Component

• GO:0005737 cytoplasm
• GO:0030430 host cell cytoplasm
• GO:0042025 host cell nucleus

External links

• PDB: RCSB:7qtl, PDBe:7qtl, PDBj:7qtl
• PDBsum: 7qtl
• PubMed: 36596301
• UniProt: M9TLW3